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陈志平, 刘瑞清, 李崇云, 王应祥. 1996: 三种姬鼠的染色体比较研究. 动物学研究, 17(3): 347-352.
引用本文: 陈志平, 刘瑞清, 李崇云, 王应祥. 1996: 三种姬鼠的染色体比较研究. 动物学研究, 17(3): 347-352.
CHEN Zhi-ping, LIU Rui-qing, LI Chong-yun, WANG Ying-xiang. 1996. Studies on The Chromosomes of Three Species of Wood Mice. Zoological Research, 17(3): 347-352.
Citation: CHEN Zhi-ping, LIU Rui-qing, LI Chong-yun, WANG Ying-xiang. 1996. Studies on The Chromosomes of Three Species of Wood Mice. Zoological Research, 17(3): 347-352.

三种姬鼠的染色体比较研究

Studies on The Chromosomes of Three Species of Wood Mice

  • 摘要: 本文采用染色体分带技术(G-,C-带和银染色),对中华姬鼠 (Apodemus draco)、大林姬鼠(A. peninsulae) 和大耳姬鼠(A.latronum)的核型进行了观察分析。结果表明:3种姬鼠的2n均为48。中华姬鼠的染色体均为端着丝点染色体。大林姬鼠的常规核型中,除1对中着丝点染色体No.23)外,其余均为端着丝点染色体。大耳姬鼠的核型中,有13对端着丝点染色体,2对亚端着丝点染色体,1对亚中着丝点染色体和7对中着丝点染色体。中华姬鼠C-带核型中,所有染色体着丝点C-带都呈强阳性,异染色质非常丰富,Y染色体整条深染。在大林姬鼠C-带核型中,Nos.7,11,15,21,22着丝点C-带弱化甚至近阴性,其余染色体着丝点异染色质C-带都呈现程度不同的阳性。且Nos.2,4,7有强弱不同的端位异染色质带。X染色体着丝点区有大块的异染色质斑带出现,Y染色体整条深染。大耳姬鼠除Nos.3,4,10,12,13染色体着丝点C-带很弱外,其余染色体着丝点C-带均呈阳性,并有8对(Nos.16—23)染色体出现异染色质短臂。从总体上看,大林姬鼠和大耳姬鼠的着丝点异染色质明显比中华姬鼠的少。中华姬鼠的Ag-NORs有5对,分别位于Nos.7,8,18,21,22染色体端部。大林姬鼠和大耳姬鼠的Ag-NORs均为2对,位于Nos.7,8染色体端部,其中No.7染色体的Ag-NORs在绝大部分观察分裂相中都呈现多态性。3种姬鼠的染色体均显示比较清晰的G-带。通过核型的比较分析,对3种姬鼠的分类地位进行了讨论。

     

    Abstract: The karyotypes of three species of wood mice have been studied by banding techniques (G-,C- and Ag-staining).Three species have the same diploid number,2n=48.All chromosomes in the complement of A.draco are acrocentrics.The karyotype of A.peninsulae consists of 22 pairs of acrocentrics,1 pair of metacentrics,X and Y are acrocentrics.However,the karyotype of A.latronum consists of 13 pairs of acroentrics,2 pair of subacrocentrics,1 pair of submetacentric,7 pairs of metacentrics,X chromosome is a acrocentric.The G-banded,C-banded and silver-stained karyotypes have been observed.G-banding allows to identify all chromosome pairs in three species.C-banding reveals centromeric heterochromatin in all chromosomes in A.draco.In the C-banded karyotype of A.peninsulae,the centromeric heterochromatin is found in all chromosomes,however,the centromeric C-bands of Nos.7,11,15,21,22 are very fanitly stained even negative,there are also telomeric C-bands in Nos.2,4,7.The Y chromosomes of A.draco and A.peninsulae are whole heterochromatin.The centromeric C-bands of all chromosomes are positive in the C-banded karyotype of A.latronum,but the centromeric C-bands in Nos.3,4,10,12,13 are very fanitly stained.Ag-NORs are predominantly located at Nos.7,8,18,21,22 in A.draco.While Ag-NORs are found in Nos.7,8 in A.peninsulae and A.latronum,respectively.The polymorphism of Ag-NORs of No.22 (in A.draco) and No.7 (in A.peninsulae and A.latronum) is found.Moreover,the classification of three species has also been discussed.

     

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