Abstract: Forty male SD rats were randomly divided into five groups：normal、model、Vitamin E、Selenium and a combination group. Rats were fed with an improved high fat diet, but a different diet, with the addition of Vitamin E (250 mg/kg diet)，Selenite (0.5 mg/kg diet) or both combined, was used in the intervention groups. All subjects were terminated at the end of the fifth week. Pathological changes in rat livers were observed. TG, TC, ALT, AST, MDA, SOD in serum and expression of UCP2 mRNA and protein in liver tissue were detected. We also studied the effects of Vitamin E and Selenium on UCP2 and other correlation factors on the rat model of non-alcoholic fatty liver. The results show the levels of TG, TC in the combination group decreased significantly, but the level of SOD activity increased. Expression of UCP2 mRNA and protein in rat liver tissue decreased. The conclusion we reached was that a combination of Vitamin E and Selenium lowers the levels of ROS, LPO and UCP2 expression, with beneficial results.