Abstract: In this exploration,agarose isoelectrofocusing,SDS-Polyacrylamide gel electrophoresis and two- dimensional electrophoresis method by their combination were used for the comparative study of introspecific evolutionary relationship of the wild crucian and five varieties of goldfishes.The results as follows.(1) A great quantity of the muscle proteins of the wild crucian and the goldfishes are similar,which verifies the inference that goldfishes were evolved from the wild crucian.(2) In course of the evolution from the wild crucian to goldfishes,appeared some component parts of proteins,and some disappeared,and moreover,there is a tendency of this evolution,golden crucian being the transitional link.(3) Its shown by the results of the cluster analyses and patterns of electrophoreses that the wild crucian has evoled,first to golden crucian,second to grass goldfish.Through these two links,it has evoled to wen goldfishes,and at last,two close groups.dragon-eye goldfishes (such as red dragon-eye goldfish) and oval goldfishes (such as red-head oval goldfish) have emerged.