猪獾和黄鼬mtDNA物理图谱及位点变异性初探

Studies on The Mitochondrial DNA Restriction Maps and Restriction Cites Variation of Hog-Badger and Siberian Weasel

摘要: 本实验用ApaⅠ，BglⅠ，Bg1Ⅱ，ClaⅠ，EcoRⅠ，EcoRV，HindⅢ，HpaⅠ，PstⅠ，PvuIⅡ，SacⅠ，SalⅠ等12种限制性内切酶分析猪獾和黄鼬的mtDNA限制性片段，并用双酶解法构建限制性内切酶图谱。结合以往积累的资料，我们对哺乳动物mtDNA限制性位点在远缘物种间的保守性和变异性进行了初步讨论。

Abstract: The mitochondrial DNAs (mtDNAs) from hog-badger (Arctonyx collaris) and siberian weasel (Mustela sibirica) liver were digested with 12 restriciton endonucleases,Apa I,Bgl Ⅰ,Bgl Ⅱ,Cla Ⅰ,EcoR Ⅰ,EcoR V,Hind Ⅲ,Hpa Ⅰ,Pst Ⅰ,Pvu IⅡ,Sca Ⅰ,Sal Ⅰ.The mtDNAs were also cleaved by double-enzyme digestions with the above mentioned enzymes.Restriction maps for hog-badger and siberian weasel were made according to the number and size of those fragments obtained by single and doubledigestions.Combining the previous data,the conservation and variation of mtDNA restriction sites of mammals among far ranged species were also discussed.