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宁应之, 沈韫芬. 1996: 珞珈山森林土壤原生动物生态学研究及土壤原生动物定量方法探讨. 动物学研究, 17(3): 225-232.
引用本文: 宁应之, 沈韫芬. 1996: 珞珈山森林土壤原生动物生态学研究及土壤原生动物定量方法探讨. 动物学研究, 17(3): 225-232.
NING Ying-zhi, SHEN Yun-fen. 1996. Ecologicol Studies on The Forest Soil Protozoa of Mount Luojiashan and Exploration of Quantitative Methods For Soil Protozoa. Zoological Research, 17(3): 225-232.
Citation: NING Ying-zhi, SHEN Yun-fen. 1996. Ecologicol Studies on The Forest Soil Protozoa of Mount Luojiashan and Exploration of Quantitative Methods For Soil Protozoa. Zoological Research, 17(3): 225-232.

珞珈山森林土壤原生动物生态学研究及土壤原生动物定量方法探讨

Ecologicol Studies on The Forest Soil Protozoa of Mount Luojiashan and Exploration of Quantitative Methods For Soil Protozoa

  • 摘要: 1993年1月至12月,用直接计数法和培养计数法对武昌珞珈山森林土壤原生动物进行了周年定量研究,发现原生动物丰度的周年动态趋势为:春季(3—5月)达到高峰,低谷出现在冬季(1—2月),夏秋季保持较平稳的变动。原生动物丰度与土壤环境因子间的相关性分析结果表明,原生动物丰度的周年动态与土壤含水量及土壤pH值的变化呈显著的正相关,与土壤温度的变化无明显相关关系。珞珈山森林土壤原生动物优势种为梨波豆虫(Bodo edax)、球波豆虫(Bodo globosus)、卵形隐滴虫(Cryptomonas ovata)、长尾尾滴虫(Cercomonas longicauda)、线条三足虫(Trinema lineare)、斜口三足虫(T.enchelys)、长匣壳虫(Centropyxis elongata)、宽口圆壳虫(Cyclopyxis eurostoma)。常见种有梨波豆虫(Bodo edax)、球波豆虫(B.globosus)、跳侧滴虫(Pleuromonas jaculans)、长尾尾滴虫(Cercomonas longicauda)、小滴虫(Monas minimum)、斜口三足虫(Trinema enchelys)、线条三足虫(T.lineare)、长匣壳虫(Centropyxis elongata)、膨胀肾形虫(Colpoda inflata)、齿脊肾形虫(C.steinii)、僧帽肾形虫(C.cucullus)、有肋薄咽虫( Leptopharynx costatus)、凹扁前口虫(Frontonia depressa)。两种定量方法的结果所反映的土壤原生动物丰度的周年变化趋势是一致的,但培养计数结果比直接计数结果分别大100—1000倍,一方面说明了土壤中的大多数原生动物个体以包囊形式存在,只有少数呈活动状态;另一方面说明培养计数法过高地估计了土壤中原生动物的现存量。对两种计数方法进行对比分析讨论后认为,只有直接计数法能对土壤中原生动物的数量提供较为可靠的估计,在客观条件允许的情况下,以采用直接计数法为佳。

     

    Abstract: The forest soil protozoa of Mount Luojia was studied quantitatively with both direct and culture counting methods from January to December in 1993.It was dicovered that the summit of the annual abundance dynamics of protozoa was in spring (March,April & May),the valley in winter (January & Febrary),and the abundance fluntuated gently in summer and fall.The results of regression analyses showed that there were obvious positive correlations between the annual dynamics of protozoan abundance and the variations of water amount and Ph value of the soil,and there was no obvious correlation between the dynamics and the variations of the soil temperature.The dominant species were Bodo edax,Trinema lineare,T.enchelys, B.globosus,Centropyxis elongata,Cryptomonas ovata,Cyclopyxis eurostoma and Cercomonas longicauda.The common species were B.edax,B.globosus,Pleuromonas jaculans,T.lineare,T.enchelys,Centropyxis elongata,Colpoda inflata,C.steinii,Leptopharynx costatus,Frontonia depressa,Cercomonas longycauda,C.cucullus and Monas minimum.The variation trend of protozoan abundance showed by the two counting methods was consistent,but the results of the culture method were 102 to 103 times as much as the ones of the direct counting method.This demonstrated that most of protozoan individuals existed in cyst forms and only small numbers in active forms in soil,and on the other hand,the culture counting method overestimated the standing crop of soil protozoa.Therefore,we believed that direct counting method gives more reliable estimations of the abundance of soil protozoa and it is better to adopt direct counting method if the objective conditions permit.

     

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