沼泽水牛与摩拉水牛杂种一代精母细胞联会复合体分析

Analysis of Synaptonemal Complexes in Spermatocytes of the Hybrid F1 Between Swamp and Murrah Buffalo

摘要: 应用界面铺张——硝酸银染色技术，对沼泽水牛和摩拉水牛杂种一代精母细胞联会复合体（SC）进行电镜观察。结果表明，在减数分裂粗线期，杂种水牛精毋细胞中形成22条正常的常染色体SC，一个中着丝粒/亚中着丝粒/端着丝粒三价体和XY双价体。这表明沼泽水牛和摩拉水牛的染色体具有高度的同源性，沼泽水牛1号染色体是由摩拉水牛4号、9号染色体串联易位而形成。

Abstract: The diploid chromosome number of hybrid F1 between Swamp and Murrah buffalo is 49.It is known that the chromosomal banding pattern of the two forms of buffalo are quite similar.No.1 chromosome of Swamp may result from a telomere-centromere tandem translocation between No.4 and No.9 chromosomes in the Murrah karyotype.In order to confirm this explanation the synaptonemal complex in spermatocytes of F1 hybrid has been examined by electron microscopy.At pachytene,22 autosomal SCs,an metacentric/submetacentric/telocentric trivalent and an XY pair were observed.Our results indicate that No.1 chromosome of Murrah could be result of a tandem translocation.There is high chromosomal homology betweem the two buffalos.