Abstract: The karyotypes of 10 species in Sylviinae and 4 species in Muscicapinae were studied with colchicine-hypotonic-air drying technique from bone marrow cells.The flycatchers had the subtelocentric or telocentric Z chromosome,which showed that their karyotypes were primitive than Warblers.Goldcrest had more metacentrics,which showed their karyotypes were specialized than Warblers.Sibley et al.(1988) had placed these three groups into different families by DNA-DNA hybridization data,the karyotype data seem supporting this classification.