贵州北部四种丙栖类乳酸脱氢酶同工酶的比较研究

Ldh Isozyme Comparative Studies of Various Tissues in Four Species of Amphibians

摘要: 本文采用聚丙烯酰胺凝胶电泳方法，研究了贵州北部的四种两栖类动物：细痣疣螈、棘指角蟾、绿臭蛙和小弧斑姬蛙的心肝、肾等组织的乳酸脱氢酶（LDH）同工酶，发现LDH具有明显的种族特异性和组织特异性，不同种的动物和同种动物的不同组织都有各自的LDH同酶谱型，可以用它们作为识别物种的附加指标。本文初步探讨了生化分类学与系统分类学的关系。

Abstract: This paper dealt with LDH isozymes of heart,liver,kidney of amphibians:Tylototriton asperrimus,Megophrys spinatus,Rana margaratae and Microhyla heymonsi by using polyacrylamide gel egectrophoresis and optical density scanning.There were different type LDH isozymes in each tissues of all animals.The results indicated that LDH isozymes had tissue specificity and different LDH zymograms in each tissue of the animals.Animals may be identified by special zymograms.The LDH molecule evolution and LDH relationship with systematics were discussed too.