Abstract: The karyotype of Ichthyophis bannanica has been studied by using the preparations of its peripheral lymphocytes.The diploid number is 2n=42.The fundamental number (NF) of chromosome arms is 62.The relative length,arm ratio,and centromere position of each chromosome are listed in table 1 and illustrated in figure 1.Chromosomes 1 and 2 are easily distinguished by their large size and metacentric morphology.No.3 is also readily identified as it is a large bi-armed chromosome with one arm visibly shorter than the other.Compared the karyotype of I.bannanica with the karyotype of I.glutinosus,both species have a karyotype with 2n=42,whereas NF=62 in I.bannanica,NF=60 in I.glutinosus,chromosomes 3 and 11 are submetacentrics in I.abnnanica,but are telocentrics in I.glutinosus.