Abstract: Phylogenetic relationships of 8 genera of Megophryidae (Anura) were discussed based on 25 partial sequences of mitochondrial 16 S rRNA gene.Maximum likelihood,maximum parsimony,and neighbor joining analyses were performed on the dataset.The ingroup was divided into two major clades,Clade 1 and Clade 2,on the gene tree.Clade 1 included Megophrys,Brachytarsophrys and Atympanophrys,while Leptolalax,Oreolalax,Scutiger,Leptobrachium and Vibrissaphora belonged to Clade 2.This met the result of morphological study.It was primarily estimated that the divergence age of two clades occurred 14.2-18.7 million years ago.The topology of Clade 1 did not support Megophrys to be a monophyletic group.In Clade 2,Leptolalax was believed to be a valid genus and was the taxon that occurred much early.