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Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis)

Xiao-Hong LIU Yong-Gang YAO

Xiao-Hong LIU, Yong-Gang YAO. Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis). Zoological Research, 2013, 34(E2): 13362-E. doi: 10.3724/SP.J.1141.2013.E02E62
Citation: Xiao-Hong LIU, Yong-Gang YAO. Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis). Zoological Research, 2013, 34(E2): 13362-E. doi: 10.3724/SP.J.1141.2013.E02E62

Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis)

doi: 10.3724/SP.J.1141.2013.E02E62
基金项目: This work was supported by the National 863 Project of China (2012AA021801) and grants from Chinese Academy of Sciences (KSCX2-EW-R-11 and KSCX2-EW-J-23) and Yunnan Province (2009CI119)

online supplementary file

    Yong-Gang YAO

Characterization of 12 polymorphic microsatellite markers in the Chinese tree shrew (Tupaia belangeri chinensis)

  • 摘要: The Chinese tree shrew (Tupaia belangeri chinensis) is a small experimental animal with a close affinity to primates. This species has long been proposed to be an alternative experimental animal to primates in biomedical research. Despite decades of study, there is no pure breed for this animal, and the overall genetic diversity of wild tree shrews remains largely unknown. In order to obtain a set of genetic markers for evaluating the genetic diversity of tree shrew wild populations and tracing the lineages in inbreeding populations, we developed 12 polymorphic microsatellite markers from the genomic DNA of the tree shrew. An analysis of a wild population of 117 individuals collected from the suburb of Kunming, China, showed that these loci exhibited a highly expected heterozygosity (0.616). These 12 microsatellites were sufficient for individual identification and parentage analysis. The microsatellite markers developed in this study will be of use in evaluating genetic diversity and lineage tracing for the tree shrew.
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  • 收稿日期:  2012-11-18
  • 修回日期:  2012-12-20
  • 刊出日期:  2013-04-08