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DNA barcoding analysis of processed medicinal insect Catharsius molossus

WANG Cheng-Ye FENG Ying CHEN Xiao-Ming

WANG Cheng-Ye, FENG Ying, CHEN Xiao-Ming. DNA barcoding analysis of processed medicinal insect Catharsius molossus. Zoological Research, 2012, 33(6): 597-602. doi: 10.3724/SP.J.1141.2012.06597
Citation: WANG Cheng-Ye, FENG Ying, CHEN Xiao-Ming. DNA barcoding analysis of processed medicinal insect Catharsius molossus. Zoological Research, 2012, 33(6): 597-602. doi: 10.3724/SP.J.1141.2012.06597

药用昆虫神农洁蜣螂炮制品DNA条形码序列的测定与分析

doi: 10.3724/SP.J.1141.2012.06597
基金项目: 云南省应用基础研究面上项目(2011FB141); 中央级公益性科研院所基本科研业务费专项 (riricaf200904m-2)
详细信息
  • 中图分类号: Q915.819+.7

DNA barcoding analysis of processed medicinal insect Catharsius molossus

  • 摘要: 当药用昆虫被加工炮制成碎块或粉末状态时,其昆虫类别和真伪的鉴别就显得十分困难.未经准确鉴定的药用昆虫会影响临床用药安全.为寻找准确、灵敏且快速的检测方法,采用DNA条形码技术, 分别以药用神农洁蜣螂 (Catharsius molossus) 炮制品药材的完整和破碎个体为研究材料, 对其mtCOI基因进行提取、扩增及测序分析, 探讨DNA条形码技术对炮制后药用蜣螂鉴定的可行性.通过对不同DNA提取方法的比较, 成功从炮制后干燥药材虫体内提取出总DNA并扩增出mtCOI基因片段.测序分析结果表明mtCOI基因序列能够区分神农洁蜣螂和其形态近似种.作为药用神农洁蜣螂DNA条形码标准片段的mtCOI基因序列已投递至GenBank.研究还表明, 碎块状药用蜣螂药材中混杂有一定比例的其他昆虫.该结果有助于建立适合加工炮制后药用蜣螂的DNA条形码分子鉴定标准, 并为保证临床用药安全提供支持.
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  • 收稿日期:  2012-07-05
  • 修回日期:  2012-09-10
  • 刊出日期:  2012-12-08

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