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Effects of three antigens extracted from Vibrio vulnificus on the immunological protection of Nibea albiflora

YAN Mao-Cang SHAN Le-Zhou CHEN Shao-Bo XIE Qi-Lang

YAN Mao-Cang, SHAN Le-Zhou, CHEN Shao-Bo, XIE Qi-Lang. Effects of three antigens extracted from Vibrio vulnificus on the immunological protection of Nibea albiflora. Zoological Research, 2012, 33(5): 503-509. doi: 10.3724/SP.J.1141.2012.05503
Citation: YAN Mao-Cang, SHAN Le-Zhou, CHEN Shao-Bo, XIE Qi-Lang. Effects of three antigens extracted from Vibrio vulnificus on the immunological protection of Nibea albiflora. Zoological Research, 2012, 33(5): 503-509. doi: 10.3724/SP.J.1141.2012.05503

三种创伤弧菌免疫原的制备及其对黄姑鱼的免疫保护效果

doi: 10.3724/SP.J.1141.2012.05503
基金项目: 浙江省科技计划项目(2006R20001); 温州市科技计划项目(S20070051); 浙江省科技计划项目(2007F10009); 温州市重点科技创新团队建设项目(C20120004); 浙江省科技计划项目(2009C12081); 浙江省科技厅创新团队建设与人才培养项目(2011F20032)
详细信息
  • 中图分类号: Q959.483; S942

Effects of three antigens extracted from Vibrio vulnificus on the immunological protection of Nibea albiflora

  • 摘要: 从创伤弧菌(Vibrio vulnificus)中提取菌体脂多糖(lipopolysaccaride, LPS)和外膜蛋白(outer membrane protein, OMP)并制备福尔马林灭活全菌苗(FKC), 腹腔注射接种黄姑鱼。分别在注射第0、7、14、21和28天后测定了受免鱼血清中凝集抗体效价、血清溶菌酶活性和血液白细胞吞噬活性, 以及免疫28 d后的相对免疫保护率。结果表明, 3种抗原对黄姑鱼均有较强的免疫原性。免疫后, 免疫组血清凝集抗体效价逐渐增高, 第28天时最高; 溶菌酶活性(LMZ)、白细胞吞噬活性(PP)和吞噬指数(PI)显著升高(P<0.01), 第21天达到峰值, 随后逐渐下降。各组之间比较表明, 受免后7、14、21和28 d, 免疫组黄姑鱼凝集抗体效价、PP、PI和LMZ显著高于对照组(P<0.05), LPS和OMP组凝集抗体效价低于FKC组, LPS和OMP组的相对免疫保护率高于FKC组, 各组间免疫保护率大小顺序为LPS组>OMP组>FKC组>对照组。
  • [1] Bader JA, Shoemaker CA, Klesius PH. 2004. Immune response induced by N-lauroylsarcosine extracted outer-membrane proteins of an isolate of Edwardsiella ictaluri in channel catfish [J]. Fish Shellfish Immunol, 2004, 16(3): 415-428.
    [2] Bøgwald J, Stensvag K, Hoffman J, Holm KO, Jørgensen Tø. 1992. Vaccination of Atlantic salmon, Salmo salar L., with particulate lipopolysaccharide antigens from Vibrio salmonicida and vibrio anguillarum [J]. Fish Shellfish Immunol, 2(4): 251-256.
    [3] Chen CF, Li J, Zhou WH, Zhao GZ. 1996. Studies on the immunoprophylaxis against bacterial septicemia in Mandarin fish [J]. J Huazhong Agric Univ, 15(5): 475-479. [陈昌福, 李静, 周文豪, 赵桂珍. 1996. 翘嘴鳜细菌性败血症的免疫预防研究. 华中农业大学学报, 15(5): 475-479.]
    [4] Dalmo RA, Kjerstad AA, Arnesen SM, Tobias PS, Bøgwald J. 2000. Bath exposure of Atlantic halibut (Hippoglossus hippoglossus L.) yolk sac larvae to bacterial lipopolysaccharide (LPS): Absorption and distribution of the LPS and effect on fish survival [J]. Fish Shellfish Immunol, 10(2): 107-128.
    [5] Ingil? M, Arnesen J A, Lund V, Eggset G. 2000. Vaccination of Atlantic halibut Hippoglossus hippoglossus L., and spotted wolffish Anarhichas minor L., against atypical Aeromonas salmonicida [J]. Aquaculture, 183(1-2): 31-44.
    [6] Huang XX, Lu CP. 2002. Comparative evaluation of the immune protection of Edwardsiella tarda whole cell bacteria and its OMPs [J]. Immunol J, 18(3): 187-189. [黄新新, 陆承平. 2002. 迟缓爱德华氏全菌及外膜蛋白的免疫力比较. 免疫学杂志, 18(3): 187-189.]
    [7] Jian JC, Wu ZH, Chen G, Zeng ML. 2003. Isolation and characteristics of pathogen of Vibriosis in cobia (Rachycentron canadum) Maricultured in cage [J]. Chn J Vet Sci, 23(4): 329-330. [简纪常, 吴灶和, 陈刚, 曾敏玲. 2003. 海水网箱养殖军曹鱼弧菌病病原的分离及其特性 [J]. 中国兽医学报, 23(4): 329-330.]
    [8] Jian JC, Ye JM, Wu ZH. 2004. Influence of lipopolysaccharide from Vibrio alginolyticus on immunological function of Epinephelus Skaara [J]. Acta Hydrobiol Sin, 28(1): 103-105. [简纪常, 叶剑敏, 吴造和. 2004. 溶藻弧菌脂多糖对石斑鱼免疫功能的影响 [J]. 水生生物学报, 28(1): 103-105.]
    [9] Jung CR, Park MJ, Heo MS. 2005. Immunization with major outer membrane protein of Vibrio vulnificus elicits protective antibodies in a murine model [J]. J Microbiol, 43(5): 437-442.
    [10] Kawai K, Kusuda R. 1995. Listonella anguillarum infection in ayu, Plecoglossus altivelis, and its prevent ion by vaccination [J]. Israeli J Aquacul, 47(3-4): 173-177.
    [11] Kawai K, Liu Y, Ohnishi K, Oshima S. 2004. A conserved 37 kDa outer membrane protein of Edwardsiella tarda is an effective vaccine candidate [J]. Vaccine, 22(25-26): 3411-3418.
    [12] Luo L, Chen XX, Cai XF. 2001. Influence of Creat (Andrographis Paniculata) on the phagocytic activity of phagocyies in Grass Carp [J]. J Southwest Agric Univ, 2001, 23(1): 33-34, 38. [罗琳, 陈孝煊, 曹雪峰. 2001. 穿心莲对草鱼血液吞噬细胞吞噬活性的影响 [J]. 西南农业大学学报, 23(1): 33-34, 38.]
    [13] Lutwyche P, Exner MM, Hancock REW, Trust TJ. 1995. A conserved Aermonas salmonicida porin provides protective immunity to rainbow trout [J]. Infect Immunol, 63(8): 3137-3142.
    [14] Ma AM, Yan MC, Chang WS, Chen SB, Shan LZ, Xie QL. 2008. Isolation and identification of Vibrio vulnificus from diseased Nibea albiflora [J]. Chn J Zoon, 24(10): 960-964. [马爱敏, 闫茂仓, 常维山, 陈少波, 单乐州, 谢起浪. 2008. 黄姑鱼创伤弧菌的分离和鉴定 [J]. 中国人兽共患病学报, 24(10): 960-964.]
    [15] Ma AM, Yan MC, Hu LH, Xie QL, Chen SB, Dan LZ. 2010. Study on immune efficacy induced by killed Vibrio splendidus vaccine on Sciaenops ocellatus [J]. J Hydroecol, 3(3): 137-141. [马爱敏, 闫茂仓, 胡利华, 谢起浪, 陈少波, 单乐州. 2010. 灿烂弧菌灭活疫苗对美国红鱼免疫效果的研究. 水生态学杂志, 3(3): 137-141.]
    [16] Maji S, Mali P, Joardar SN. 2006. Immunoreactive antigens of the outer membrane protein of Aeromonas hydrophila, isolated from goldfish, Carassius auratus (Linn.) [J]. Fish Shellfish Immunol, 20(4): 462-473.
    [17] Mao ZJ, You ZQ, Wei YW, Yu L. 2007a. Cloning, expression and immunogenicity analysis of two iron-regulated outer membrane proteins of Vibrio parahaemolyticus ZJ2003 [J]. J Fish Sci Chn, 14(4): 563-569. [毛芝娟, 由振强, 魏永伟, 于涟. 2007a. 副溶血弧菌ZJ2003 株两种铁调外膜蛋白的克隆、表达和免疫原性. 中国水产科学, 14(4): 563-569.
    [18] Mao ZJ, Yu L, You ZQ, Wei YW, Liu Y. 2007b. Cloning, expression and immunogenicty analysis of five outer membrane proteins of Vibrio parahaemolyticus zj2003 [J]. Fish Shellfish Immunol, 23(3): 567-575.
    [19] Meng XL, Chen CF, Wu ZX, Luo YL. 2009. Comparative study on immunogenicity of three vaccines of Aeromonas hydrophila to channel catfish, Ictalurus punctatus [J]. Freshwat Fish, 39(4): 49-53. [孟小亮, 陈昌福, 吴志新, 罗宇良. 2009. 嗜水气单胞菌3种疫苗对斑点叉尾鮰免疫原性比较研究. 淡水渔业, 39(4): 49-53.]
    [20] Mo ZL, Xu YL, Zhang PJ. 2002. Vaccination Against Vibrio Anguillarum on cultured flounder, Paralichthys olivaceus [J]. Marine Sci, 26(4): 62-66. [莫照兰, 徐永立, 张培军. 2002. 养殖牙鲆鳗弧菌疫苗的研究. 海洋科学, 26(4): 62-66.]
    [21] Rahman MH, Kawai K. 2000. Outer membrane proteins of Aeromonas hydrophila induce protective immunity in goldfish [J]. Fish Shellfish Immunol, 10(4): 379-382.
    [22] Reed L J, Muench H. 1938. A simple method of estimating fifty percent end points [J]. Am J Epidemiol, 27(3): 493-497.
    [23] Shen ZQ, Liu JS, Li F, Xu KL, Wang ZJ, Lu CP, Chu WH. 2001. Isolation and identification of pathogen in stone flounder with Vibrio vulnificus infection as well as treatment and prevention for the disease [J]. Chn J Vet Sci Technol, 31(10): 22-23. [沈志强, 刘吉山, 李锋, 徐可利, 王忠军, 陆承平, 储卫华. 2001. 石鲽鱼创伤弧菌病的病原分离鉴定与防治. 中国兽医科技, 31(10): 22-23.]
    [24] Solem ST, Jørgensen JB, Roberrtsen B. 1995. Stimulation of respiratory burst and phagocytic activity in Atlantic salmon (salmo salar L.) macrophages by lipopolysaccharide [J]. Fish Shellfish Immunol, 5(7): 475-491.
    [25] Westphal O, Jann K. 1969. Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure [J]. Meth in Carbohyd Chem, 5: 83-96.
    [26] Xu BF, Lin TL, Dong CF, Yi GH. 2005. Molecular identification of Vibrio vulnificus isolated from the diseased European eels in mainland China [J]. Chn J Zoon, 21(11): 995-997, 980. [许斌福, 林天龙, 董传甫, 伊光辉. 2005. 鳗鲡创伤弧菌的分子鉴定. 中国人兽共患病杂志, 21(11): 995-997, 980.]
    [27] Yan MC. 2005. Study on Outer Membrane Proteins and Lipopolysaccrides of Vibrio Harveyi [D]. Master thesis, Huazhong Agricultural University, 32-40. [闫茂仓. 2005. 哈氏弧菌外膜蛋白和脂多糖的研究[D]. 硕士论文, 华中农业大学, 32-40.]
    [28] Yan MC, Chen SB, Shan LZ, Wang XP, Ma AM, Xie QL. 2010. Comparison of different preservation methods for Vibrio splendidus and Vibrio vulnificus [J]. J Hydroecol, 3(5): 136-140. [闫茂仓, 陈少波, 单乐州, 王雪鹏, 马爱敏, 谢起浪. 2010. 灿烂弧菌和创伤弧菌的不同保存方法比较. 水生态学杂志, 3(5): 136-140.]
    [29] Yang GW, An LG, Wang CF, Wen WJ. 1998. Comparative studies on the immunoglobulin in skin mucus nad serum of carp [J]. Zool Res, 19(6): 489-492. [杨桂文, 安利国, 王长法, 温武军. 1998. 鲤鱼皮肤粘液与血清中免疫球蛋白的比较研究. 动物学研究, 19(6): 489-492.]
    [30] Ye JM, Jian JC, Wu HB, Wu ZH. 2004. Chemical composition of lipopolysaccharides (LPS) from Vibrio alginolyticus and toxicity of LPS on Epinephelus akaara [J]. Acta Hydrobiol Sin, 28(5): 574-576. [叶剑敏, 简纪常, 吴后波, 吴灶和. 2004. 溶藻弧菌脂多糖的化学成分分析及其对石斑鱼的毒性. 水生生物学报, 28(5): 574-576.]
    [31] Yu LP, Wang B, Li Y, Hu L, Zhao FM. 2008. Isolation and identification of haemorrihagic septicaemia pathogen from cultivated turbot Scophthalmus maximus [J]. J Dalian Fish Univ, 23(5): 335-339. [于兰萍, 王斌, 李艳, 胡亮, 赵凤梅. 2008. 大菱鲆出血性败血症病原菌的分离与鉴定. 大连水产学院学报, 23(5): 335-339.]
    [32] Zhang B, Zeng LB, Luo XS, Gao ZY, Zhou Y, An W. 2012. Responses of immune parameters in periphery blood of black carp (Mylopharyngodon piceus) immunized with three vaccines of Aeromonas hydrophila [J]. J Huazhong Agric Univ, 31(1): 100-105. [张波, 曾令兵, 罗晓松, 高正勇, 周勇, 安伟. 2012. 嗜水气单胞菌3种疫苗免疫的青鱼外周血免疫指标的变化. 华中农业大学学报, 31(1): 100-105.]
    [33] Zhang YA, Nie P. 2009. Humoral immune factors of fish: a review [J]. J Fish Chn, 24(4): 376-381. [张永安, 聂品. 2000. 鱼类体液免疫因子研究进展. 水产学报, 24(4): 376-381.]
    [34] Zhang YN, Li XY, Geng XN, Zhao BH. 2011. Cloning and expression of a outer membrance protein gene (OMPs2) of Edwardsiella tarda HB01 and its immunogenicity [J]. Chn J Cell Mol Immunol, 27(10): 1075-1079. [张亚宁, 李晓玥, 耿晓娜, 赵宝华. 2011. 迟缓爱德华菌HB01外膜蛋白OmpS2基因的克隆表达及其免疫原性研究. 细胞与分子免疫学杂志, 27(10): 1075-1079.]
    [35] Zhao DH, Liu LB, Sun JJ, Wang HF, Liang LQ, Zhao HH, Liu L, Li G F. 2007. Histopathology of ovate pompano Trachinotus ovatus infected with Vibrio damsela and Vibrio vulnificus [J]. J Dalian Fish Univ, 22(6): 455-459. [赵典惠, 刘丽波, 孙际佳, 王海芳, 梁兰清, 赵会宏, 刘丽, 李桂峰. 2007. 用美人鱼弧菌与创伤弧菌人工感染卵形鲳鲹的组织病理学研究. 大连水产学院学报, 22(6): 455-459.]
    [36] Zhao F. 2005. Effects of Rapeseed Meal on the Immune Function of Carassius Auratus Gibelio [D]. Master thesis, Huazhong Agric Univ, 18-19. [赵飞. 2005. 菜籽粕对异育银鲫免疫机能的影响[D]. 硕士论文, 华中农业大学, 18-19.]
    [37] Zhou YC, Zhang B, Chen XF, Qian JY. 2002. Preparation of Pseudomonas maltophilia lipopolysaccharidae and immunological analysis of the lipopolysaccharide against Trachinotus ovatus [J]. J Fish Chn, 26(2): 143-148. [周永灿, 张本, 陈雪芬, 钱家英. 2002. 嗜麦芽假单胞菌脂多糖的置备及其在卵形鲳鯵中的免疫反应. 水产学报, 26(2): 143-148.]
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