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Analysis of genetic diversity in wild populations of Trachidermus fasciatus by RAPD and the transformation of two SCAR markers

ZENG Zhen LIU Zhi-Zhi PAN Lian-De TANG Wen-Qiao WANG Qian GENG Yun-Hao

ZENG Zhen, LIU Zhi-Zhi, PAN Lian-De, TANG Wen-Qiao, WANG Qian, GENG Yun-Hao. Analysis of genetic diversity in wild populations of Trachidermus fasciatus by RAPD and the transformation of two SCAR markers. Zoological Research, 2012, (2): 203-210. doi: 10.3724/SP.J.1141.2012.02203
Citation: ZENG Zhen, LIU Zhi-Zhi, PAN Lian-De, TANG Wen-Qiao, WANG Qian, GENG Yun-Hao. Analysis of genetic diversity in wild populations of Trachidermus fasciatus by RAPD and the transformation of two SCAR markers. Zoological Research, 2012, (2): 203-210. doi: 10.3724/SP.J.1141.2012.02203

松江鲈鱼野生群体遗传多样性的RAPD分析和SCAR标记的转化

doi: 10.3724/SP.J.1141.2012.02203
基金项目: 上海市教育委员会重点学科(海洋生物学)建设项目(J50701); 上海市科技兴农项目(沪农科推字 2006-3-5); 上海市水生生物学重点学科建设项目(S30701); 上海市重点学科建设项目(Y1101)
详细信息
  • 中图分类号: Q959.483; Q343

Analysis of genetic diversity in wild populations of Trachidermus fasciatus by RAPD and the transformation of two SCAR markers

  • 摘要: 首先,从294条10个碱基随机引物中,筛选出32条多态性引物,对富春江、黄河、滦河和鸭绿江等4个松江鲈鱼(Trachidermus fasciatus)野生群体共120尾个体进行RAPD分析。结果表明,松江鲈鱼野生群体的遗传多样性较丰富,其主要表现在:①在扩增得到的591个位点中,有515个(87.14%)位点呈现多态性,群体间多态位点比率(P)的大小顺序为:富春江群体89.17%>黄河群体87.99%>鸭绿江群体86.63%>滦河群体83.25%。②松江鲈鱼群体间的Shannon信息指数(IT)和Nei’s遗传多样性指数(HT)分别在0.3393~0.3566和0.2157~0.2279间,滦河群体的值较其他3群体稍低; 若作为一个整体,则总的Shannon信息指数(IT)和Nei’s遗传多样性指数(HT)分别为0.3710±0.2153和0.2336±0.1643。③虽然群体间基因流值(Nm)在5.76103~19.84497间,显示各地理群体间存在程度不同的基因交流,但分子方差分析(AMOVA)结果却表明,各群体间存在显著(P<0.05)或极显著(P<0.01)的遗传分化。④聚类分析表明,鸭绿江群体首先与黄河群体聚为一支,再与富春江群体相聚,最后与单独一支的滦河群体聚类,表明鸭绿江、黄河、富春江等3群体间的遗传距离与彼此间的地理距离远近密切相关,而滦河群体与它们的遗传距离较远。其次,从获得的S1225525bp、S1225605bp、S1225841bp、S1345695bp、S1345825bp等5个特异RAPD条带中,成功地由S1225605bp、S1225841bp条带分别转化出SCAR01560bp、SCAR02443bp的SCAR标记。这两个标记的出现频率,在鸭绿江群体最高(96.67%和93.33%)、富春江群体其次(83.33%和90%)、黄河群体再其次(56.67%和66.67%)、滦河群体最低(13.33%和20%)。因此,SCAR01560bp、SCAR02443bp可作为鉴别松江鲈鱼滦河群体与其他3群体的分子标记。
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  • 收稿日期:  2011-11-03
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