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Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri)

PING Shu-Huang WANG Cai-Yun TANG Wen-Ru LUO Ying YANG Shi-Hua

PING Shu-Huang, WANG Cai-Yun, TANG Wen-Ru, LUO Ying, YANG Shi-Hua. Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri). Zoological Research, 2012, 33(1): 19-28. doi: 10.3724/SP.J.1141.2012.01019
Citation: PING Shu-Huang, WANG Cai-Yun, TANG Wen-Ru, LUO Ying, YANG Shi-Hua. Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri). Zoological Research, 2012, 33(1): 19-28. doi: 10.3724/SP.J.1141.2012.01019

几种冷冻稀释液与单胺类防冻剂对中缅树鼩精子冷冻存活率的影响

doi: 10.3724/SP.J.1141.2012.01019
基金项目: 国家自然科学基金项目(31071279, 31040055); 云南省科技创新人才计划项目(2011CI009)资助
详细信息
  • 中图分类号: Q954.43; Q959.832; Q492

Effects of some extenders and monoamines on sperm cryopreservation in tree shrews (Tupaia belangeri)

  • 摘要: 该文实验精子采自昆明地区经笼养驯化的树鼩(Tupaia belangeri), 检测其冷冻前后运动度、顶体完整率以及检测部分冷冻精子的受精能力。实验一:选用8 种已报道的冷冻稀释液TTE、TCG、TCF、TTG、BWW、BTS、DM、SR 稀释鲜精,并添加0.4 mol/L DMSO,4℃预冷平衡2h 后,TTE、DM 和SR 稀释液的精子的运动度与鲜精无差别(P>0.05),其余处理组均显著下降(P<0.05)。冷冻复苏后,各组的运动度显著低于预冷平衡处理后的运动度(P<0.05);DM 组的复苏运动度显著高于其他稀释液组(P<0.05),BWW 组最低(P<0.05)。对于顶体完整率,与鲜精相比,4℃平衡2h 后,TTE 和DM 组精子的顶体完整率显著高于BWW、BTS 和SR 组(P<0.05)。冷冻复苏后,DM 组精子的顶体完整率显著高于其它(除了TTE)冷冻组(P<0.05)。实验二:在DM 稀释液基础上分别添加4 种浓度0.2、0.4、0.8 和1.2 mol/L 的二甲基甲酰胺(DF)、甲酰胺(F)、二甲乙酰胺(DA)和乙酰胺(A)以及0.4 mol/L DMSO,经过预冷平衡处理后,与鲜精相比,各防冻剂组的精子运动度没有下降(P>0.05);冷冻解冻后,各冷冻组精子的运动度显著低于预冷平衡处理后的精子运动度(P<0.05);0.8 mol/L DF 和0.4 mol/LDMSO 组精子的运动度显著高于其它冷冻组(P<0.05)。对于顶体完整率,预冷平衡处理后各高浓度组的比率显著下降;冷冻复苏后,0.4 mol/L F 和0.4 mol/L DF 组精子的顶体完整率相对较高。实验三,人工授精实验中,DM+0.8mol/L DF 冷冻精子的受精率为16.7%,DM+0.4 mol/L DMSO 的受精率为50.0%。以上实验结果提示,含卵黄的非离子冷冻稀释液对树鼩精子冷冻保护效果好,但单胺类防冻剂的防冻效果还需进一步深入研究。
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  • 收稿日期:  2011-11-01
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