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Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis

YUE Ming-Jin MO Sai-Jun SONG Ping GONG Yan-Zhang

YUE Ming-Jin, MO Sai-Jun, SONG Ping, GONG Yan-Zhang. Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis. Zoological Research, 2011, 32(4): 386-390. doi: 10.3724/SP.J.1141.2011.04386
Citation: YUE Ming-Jin, MO Sai-Jun, SONG Ping, GONG Yan-Zhang. Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis. Zoological Research, 2011, 32(4): 386-390. doi: 10.3724/SP.J.1141.2011.04386

斑马鱼Z-OTU蛋白在卵子发生和胚胎发育早期的表达(英文)

doi: 10.3724/SP.J.1141.2011.04386
详细信息
    作者简介:

    岳明金,男,硕士研究生,主要从事动物遗传育种与繁殖研究;E-mail:ymjmsj0618@yahoo.com.cn

    通讯作者:

    宋 平, 龚炎长

Identification of Z-OTU protein during zebrafish oogenesis and early embryogenesis

Funds: This work was supported by grants from the ICGEB (International Center for Genetic Engineering and Biotechnology) (CRP/CHN02-01) (SONG Ping); the National Basic Research Program of China (2004CB117400) (SONG Ping); the National Natural Science Foundation of China (30150005; 30270675) (SONG Ping)
More Information
    Author Bio:

    YUE Ming-Jin,E-mail:ymjmsj0618@yahoo.com.cn

    Corresponding author: SONG Ping, GONG Yan-Zhang
  • 摘要: 斑马鱼z-otu基因编码的蛋白可能具有DUBs活性, 它包含OTU和TUDOR结构域, 属于OTU相关蛋白酶家族的成员。该研究将原核表达的融合蛋白(OTU结构域和GST)纯化后免疫新西兰兔, 获得多克隆抗体anti-Z-OTU, 并利用该抗体对Z-OTU蛋白质在斑马鱼卵子发生和早期胚胎发育过程中的表达进行了分析。根据原位和整体免疫组织化学检测结果并结合以前的研究结论, 分析并比较了z-otu基因的mRNA和蛋白质的分布, 发现在卵子发生和早期胚胎发育过程中, z-otu基因的mRNA和蛋白质表达模式存在明显差异:mRNA仅在卵子发生早期表达, 卵母细胞受精后才重新开始表达, 而其蛋白在卵子发生过程中均表达; 在卵子发生过程中, mRNA分布于细胞质中, 而蛋白质先分布于细胞核中, 然后向细胞质迁移, 接着又向卵母细胞生发泡(germinal vesicle, GV)集中。推测Z-OTU蛋白类似于其他具有去泛素化酶活性的OTU相关蛋白酶, 对于卵母细胞减数分裂过程中生发泡破裂、生发泡迁移及维持胚胎的分裂是必需的。
  • [1] Balakirev MY, Tcherniuk SO, Jaquinod M, Chroboczek J. 2003. Otubains: a new family of cysteine proteases in the ubiquitin pathway [J]. EMBO Rep, 4: 517-522.
    [2]  Ben-Yehoshua Josefsberg L, Kaufman O, Galiani D, Kovo M, Dekel N. 2001. Inactivation  of M-phase promoting factor at exit from first embryonic mitosis in the rat is independent of cyclin B1 degradation [J]. Biol Reprod, 64: 871-878.
    [3]  Braat AK, van de, Water S, Goos H, Bogerd J, Zivkovic D. 2000. Vasa protein expression and localization in the zebrafish [J]. Mech Dev, 95: 271-274.
    [4]  Dekel N. 1996. Protein phosphorylation/dephosphorylation in the meiotic cell cycle of mammalian oocytes [J]. Rev Reprod, 1: 82-88.
    [5]  Evans PC, Smith TS, Lai MJ, Williams MG, Burke DF, Heyninck K, Kreike MM, Beyaert R, Blundell TL, Kilshaw PJ. 2003. A novel type of deubiquitinating enzyme
    [J]. J Biol Chem, 278: 23180-23186.
    [7]  Evans PC, Ovaa H, Hamon M, Kilshaw PJ, Hamm S, Bauer S, Ploegh HL, Smith, TS. 2004. Zinc-finger protein A20, a regulator of inflammation and cell survival, has deubiquitinating activity [J]. Biochem J, 378: 727-734.
    [8]  Huo LJ, Fan HY, Zhong ZS, Chen DY, Schatten H, Sun QY. 2004. Ubiquitin–proteasome pathway modulates mouse oocyte meiotic maturation and fertilization via regulation of MAPK cascade and cyclin B1 degradation [J]. Mech Dev, 121:1275-1287.
    [9]  Jesuthasan S, Strahle U. 1997. Dynamic microtubules and specification of the zebrafish embryonic axis [J]. Curr Biol, 7: 31-42.
    [10]  Knaut H, Pelegri F, Bohmann K, Schwarz H, Nüsslein-Volhard C. 2000. Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification [J]. J Cell Biol, 149: 875-888.
    [11]  Makarova KS, Aravind L, Koonin EV. 2000. A novel superfamily of predicted cysteine proteases from eukaryotes, viruses and Chlamydia 1 pneumoniae [J]. Trends Biochem Sci, 25: 50-52.
    [12]  Mo S, Song P, Lü D, Chen Y, Zhou W, Gong W, Zhu Z. 2005. Zebrafish z-otu, a novel Otu and Tudor domain-containing gene, is expressed in early stages of oogenesis and embryogenesis [J]. Biochim Biophys Acta, 1732: 1-7.
    [13]  Murray AW, Kirschner MW. 1989. Cyclin synthesis drives the early embryonic cell cycle [J]. Nature, 339: 275-280.
    [14]  Opipari AW, Hu Jr HM, Yabkowitz R, Dixit VM. 1992. The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity [J]. J Biol Chem, 267: 12424-12427.
    [15]  Oppenheimer J. 1936. The development of isolated blastoderms of Fundulus heteroclitus [J]. J Exp Zool, 72: 247-269.
    [16]  Pelegri F. 2003. Maternal factors in zebrafish development [J]. Dev Dyn, 228: 535-554.
    [17]  Sun QY, Fuchimotoa D, Nagaia T. 2004. Regulatory roles of ubiquitin-proteasome pathway in pig oocyte meiotic maturation and fertilization [J]. Theriogenology, 62: 245-255.
    [18]  Sieburth D, Ch'ng Q, Dybbs M, Tavazoie M, Kennedy S, Wang D, Dupuy D, Rual JF, Hill DE, Vidal M, Ruvkun G, Kaplan J. 2005. Systematic analysis of genes required for synapse structure and function [J]. Nature, 436: 510-517.
    [19]  Tokumoto T, Yamashita M, Tokumoto M, Katsu Y, Horiguchi R, Kajiura H, Nagahama Y. 1997. Initiation of cyclin B degradation by the 26S proteasome upon egg activation
    [J]. J Cell Biol, 138: 1313-1322.
    [21]  Uchiyama K Jokitalo E, Kano F, Murata M, Zhang XD, Canas B, Newman R,  Rabouille C, Pappin D, Freemont P, Kondo H. 2002. VCIP135, a novel essential factor for p97/p47-mediated membrane fusion, is required for Golgi and ER assembly in vivo [J]. J Cell Biol, 159: 855-866.
    [22]  Wing SS. 2003. Deubiquitinating enzymes-the importance of driving in reverse along the ubiquitin–proteasome pathway [J]. Int J Biochem Cell Biol, 35: 590-605.
    [23]  Xu HY, Gui JF, Hong YH. 2005. Differential Expression of vasa RNA and Protein During Spermatogenesis and Oogenesis in the Gibel Carp Carassius auratus gibelio., a Bisexually and Gynogenetically Reproducing Vertebrate [J]. Dev Dyn, 233: 872-882.
    [24]  Yang Q, Hu J, Ye D, Zhao C, Song S, Gong W, Tan Z, Song P. 2010. Identification and expression analysis of two zebrafish E2F5 genes during oogenesis and development
    [J]. Mol Biol Rep, 37: 1773-1780.
    [26]  
    [27]  
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  • 收稿日期:  2010-11-08
  • 修回日期:  2011-04-27
  • 刊出日期:  2011-08-22

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