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Rat orbital frontal (orbital frontal cortex, OFC) GABA B receptor mechanisms in stress and depression

GU Chao-Yang AN Shu-Cheng

GU Chao-Yang, AN Shu-Cheng. Rat orbital frontal (orbital frontal cortex, OFC) GABA B receptor mechanisms in stress and depression. Zoological Research, 2011, 32(3): 329-336. doi: 10.3724/SP.J.1141.2011.03329
Citation: GU Chao-Yang, AN Shu-Cheng. Rat orbital frontal (orbital frontal cortex, OFC) GABA B receptor mechanisms in stress and depression. Zoological Research, 2011, 32(3): 329-336. doi: 10.3724/SP.J.1141.2011.03329


doi: 10.3724/SP.J.1141.2011.03329
基金项目: 中央高校基本科研业务费专项资金资助项目(GK200901011)

    谷朝阳, 男, 博士研究生, 主要从事神经生理学方面的研究



  • 中图分类号: Q42; R749.72

Rat orbital frontal (orbital frontal cortex, OFC) GABA B receptor mechanisms in stress and depression

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    Author Bio:

    GU Chao-Yang

    Corresponding author: AN Shu-Cheng
  • 摘要: 为了探讨眶额叶(orbital frontal cortex, OFC)GABA及其B型受体在应激性抑郁行为发生中的作用及其影响机制, 实验采用强迫游泳方法建立急性应激抑郁模型。在OFC区微量注射γ-氨基丁酸(γ-aminobutyric acid, GABA)及其B型受体阻断剂, 通过开场实验、强迫游泳方式检测动物行为学表现, 用免疫组织化学染色和Western blotting方法检测OFC区 Kalirin表达, 用高尔基染色法观察锥体细胞树突和树突棘。结果显示:强迫游泳应激引起动物抑郁样行为表现, 同时, OFC区 Kalirin阳性颗粒数及表达量显著减少, 且锥体细胞树突棘密度下降; OFC区微量注射GABA具有抗抑郁效应, 使OFC区 Kalirin表达显著升高, 锥体细胞树突棘密度增加; GABA-B型受体阻断剂CGP35348可以抑制GABA的这种效应。由此可见, 通过强迫游泳应激诱发的抑郁样的行为变化与OFC区 Kalirin表达减少和神经元树突棘密度降低有关, GABA可能通过GABA-B型受体增加OFC区 Kalirin表达, 以防止神经元退行性变化而产生抗抑郁作用。
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  • 收稿日期:  2010-08-23
  • 修回日期:  2011-01-19
  • 刊出日期:  2011-06-22