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Expression analysis of 5 hsp70 genes in Tetrahymena thermophila

FENG Li-Fang CHANG Yue YUAN Dong-Xia MIAO Wei

FENG Li-Fang, CHANG Yue, YUAN Dong-Xia, MIAO Wei. Expression analysis of 5 hsp70 genes in Tetrahymena thermophila. Zoological Research, 2011, 32(3): 267-276. doi: 10.3724/SP.J.1141.2011.03267
Citation: FENG Li-Fang, CHANG Yue, YUAN Dong-Xia, MIAO Wei. Expression analysis of 5 hsp70 genes in Tetrahymena thermophila. Zoological Research, 2011, 32(3): 267-276. doi: 10.3724/SP.J.1141.2011.03267

嗜热四膜虫五个hsp70基因的表达分析

doi: 10.3724/SP.J.1141.2011.03267
基金项目: 中国科学院知识创新项目(KSCX2-EW-G-6-4); 国家自然基金资助项目(31071993)
详细信息
    作者简介:

    冯立芳

    通讯作者:

    缪 炜

  • 中图分类号: Q959.117; Q786

Expression analysis of 5 hsp70 genes in Tetrahymena thermophila

More Information
    Author Bio:

    FENG Li-Fang

    Corresponding author: MIAO Wei
  • 摘要: 鉴定得到嗜热四膜虫13个含有完整保守结构域的hsp70基因, 对其中5个高度相似且无内含子的hsp70基因进行表达分析。在37 、39 和41 ℃热激条件下, 实时荧光定量PCR结果表明, hsp70-2基因对热激响应最敏感。在四膜虫生长、饥饿和接合生殖这3种生理或发育状态下, Microarray结果显示, hsp70-4基因恒定且高表达; 在热激条件下, hsp70-4基因的表达水平随着温度的升高而略微增加, 证实hsp70-4基因为热休克相关蛋白hsc70基因; 克隆的hsp70-4基因全长2 208 bp, 开放阅读框长1 959 bp, 编码653个氨基酸。Microarray结果提示, hsp70-3可能参与四膜虫饥饿早期(0~12 h)的耐受和接合生殖后期(6~10 h)的新大小核形成, 老大核凋亡等事件; hsp70-5可能参与四膜虫饥饿晚期(12~15 h)的耐受和接合生殖早期(0~6 h)的小核减数分裂、小核交换和原核(pronuclear)融合事件。Blast2GO分析表明, 与hsp70-3和hsp70-5共表达的基因分别参与不同的生物学过程, 进一步反映了hsp70-3和hsp70-5这两个基因在功能上是存在差异的。
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  • 收稿日期:  2010-10-13
  • 修回日期:  2011-02-22
  • 刊出日期:  2011-06-22

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