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cDNA cloning and tissue-differential expression of Na+/K+/2Cl- −cotransporter 1-α isoform in Sarotherodon melanotheron

FAN Wu-Jiang LI Si-Fa

FAN Wu-Jiang, LI Si-Fa. cDNA cloning and tissue-differential expression of Na+/K+/2Cl- −cotransporter 1-α isoform in Sarotherodon melanotheron. Zoological Research, 2010, 31(6): 601-609. doi: 10.3724/SP.J.1141.2010.06601
Citation: FAN Wu-Jiang, LI Si-Fa. cDNA cloning and tissue-differential expression of Na+/K+/2Cl- −cotransporter 1-α isoform in Sarotherodon melanotheron. Zoological Research, 2010, 31(6): 601-609. doi: 10.3724/SP.J.1141.2010.06601

萨罗罗非鱼NKCC1α基因cDNA克隆及mRNA组织表达差异

doi: 10.3724/SP.J.1141.2010.06601
基金项目: 罗非鱼行业技术体系(nycytx-48-3); 公益性行业(农业)科研专项:罗非鱼大规格鱼种规模化培育与生态养殖技术研究(nyhyzx07-044-01); 国家科技支撑计划专题:耐盐罗非鱼新品种选育(2006BAD01A1203)
详细信息
    作者简介:

    范武江, 博士在读, 研究方向 水产动物种质资源与种苗工程, E-mail:fanwujiang@hotmail.com

    通讯作者:

    李思发

  • 中图分类号: Q785; Q959.499; Q516

cDNA cloning and tissue-differential expression of Na+/K+/2Cl- −cotransporter 1-α isoform in Sarotherodon melanotheron

More Information
    Author Bio:

    FAN Wu-Jiang,E-mail:fanwujiang@hotmail.com

    Corresponding author: LI Si-Fa
  • 摘要: 在鱼类适应盐度变化的过程中, 鱼鳃是进行渗透调节的主要器官, NKCC1α基因是保持鳃丝氯细胞渗透平衡的关键离子转运器, 以1Na+:1 K+ :2Cl 的比例进行电中性转运。萨罗罗非鱼(Sarotherodon melanothern)是耐盐强的广盐性鱼类之一。该文通过快速扩增cDNA末端的方法, 首次从萨罗罗非鱼鳃丝组织中分离出NKCC1α 基因cDNA 全长序列, 其开放阅读框(ORF)包含1 151个氨基酸残基。多重比对和聚类分析结果表明, 该试验获得的基因序列与莫桑比克罗非鱼、鲑及鳗鲡的NKCC1α最为相似, 其中萨罗罗非鱼与莫桑比克罗非鱼相似性最高(99%)。预测萨罗罗非鱼NKCC1α蛋白质二级结构包含10个跨膜螺旋, 这些跨膜螺旋的氨基酸序列及其布局都非常保守; 应用实时荧光定量PCR(qRT-PCR)检测鳃、肝、肠、肾NKCC1α mRNA相对含量, 这4种组织间差异显著; 盐度显著影响NKCC1α在鳃组织中的表达, 在136盐度下NKCC1α mRNA相对表达水平为0盐度下的4.9倍, 差异极显著(P<0.001), 显示NKCC1α基因与萨罗罗非鱼的耐盐性能密切相关。
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出版历程
  • 收稿日期:  2010-09-14
  • 修回日期:  2010-11-18
  • 刊出日期:  2010-12-22

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