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Molecular cloning, sequence analysis and expression pattern of hepcidin gene in ayu (Plecoglossus altivelis)

CHEN Mei-Zhen CHEN Jiong* LU Xin-Jiang SHI Yu-Hong

CHEN Mei-Zhen, CHEN Jiong*, LU Xin-Jiang, SHI Yu-Hong. Molecular cloning, sequence analysis and expression pattern of hepcidin gene in ayu (Plecoglossus altivelis). Zoological Research, 2010, 31(6): 595-600. doi: 10.3724/SP.J.1141.2010.06595
Citation: CHEN Mei-Zhen, CHEN Jiong*, LU Xin-Jiang, SHI Yu-Hong. Molecular cloning, sequence analysis and expression pattern of hepcidin gene in ayu (Plecoglossus altivelis). Zoological Research, 2010, 31(6): 595-600. doi: 10.3724/SP.J.1141.2010.06595

香鱼hepcidin基因的克隆、序列分析及组织表达特征

doi: 10.3724/SP.J.1141.2010.06595
基金项目: 长江学者和创新团队发展计划项目(IRT0734); 宁波市科技创新公共服务平台项目(2007A31004)
详细信息
    作者简介:

    陈梅珍(1984—), 女, 浙江丽水人, 硕士生, 研究方向:水产分子生物学; Tel: 13957420181; E-mail: cmz1211@163.com

  • 中图分类号: Q785; Q959.499; Q516

Molecular cloning, sequence analysis and expression pattern of hepcidin gene in ayu (Plecoglossus altivelis)

More Information
    Author Bio:

    CHEN Mei-Zhen,Tel: 13957420181; E-mail: cmz1211@163.com.

  • 摘要: Hepcidin是一类富含半胱氨酸的抗菌肽,在鱼类非特异性免疫中起重要作用, 具有调节铁代谢的功能。该研究克隆了香鱼hepcidin基因cDNA序列, 全长763个核苷酸, 包含一个完整的开放阅读框, 推测编码一个由85个氨基酸组成的相对分子质量为9.7 k的多肽, N端24个氨基酸是信号肽序列。香鱼hepcidin的成熟肽序列由25个氨基酸组成, 含有8个半胱氨酸, 可形成4个分子内二硫键结构。系统进化树分析表明, hepcidin的物种进化关系与目前接受的物种分类关系基本一致, 香鱼hepcidin位于鱼类hepcidin簇中, 与大西洋鲑hepcidin的亲缘关系最近, 达60%。香鱼hepcidin在肝脏中表达量最大, 在脾、肾、心脏和肌肉中也有表达。实时荧光定量PCR(qRT-PCR)结果表明, 鳗利斯顿氏菌(Listonella anguillarum)感染以后香鱼肝组织中hepcidin基因mRNA表达量显著增加, 在12 h增加了8.26倍。hepcidin基因可能在香鱼抗外界病原物感染过程中起重要作用。
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  • 收稿日期:  2010-04-26
  • 修回日期:  2010-09-16
  • 刊出日期:  2010-12-22

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