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Cloning and expression pattern analysis of a lipopolysaccharide -and β-1,3-glucan-binding protein in Portunus trituberculatus

ZHANG Ying JIN Shan ZHAO Qing-Song WANG Guo-Liang YU Kai WANG Chun-Lin

ZHANG Ying, JIN Shan, ZHAO Qing-Song, WANG Guo-Liang, YU Kai, WANG Chun-Lin. Cloning and expression pattern analysis of a lipopolysaccharide -and β-1,3-glucan-binding protein in Portunus trituberculatus. Zoological Research, 2010, 31(6): 587-594. doi: 10.3724/SP.J.1141.2010.06587
Citation: ZHANG Ying, JIN Shan, ZHAO Qing-Song, WANG Guo-Liang, YU Kai, WANG Chun-Lin. Cloning and expression pattern analysis of a lipopolysaccharide -and β-1,3-glucan-binding protein in Portunus trituberculatus. Zoological Research, 2010, 31(6): 587-594. doi: 10.3724/SP.J.1141.2010.06587

三疣梭子蟹脂多糖- β-1,3-葡聚糖结合蛋白基因的克隆及表达模式分析

doi: 10.3724/SP.J.1141.2010.06587
基金项目: 教育部创新团队项目(IRT0734); 宁波市科技局项目(2008A610073; 2008C11001); 宁波大学研究生科研创新基金资助
详细信息
    作者简介:

    张樱 (1985—), 女, 硕士研究生, 主要从事海洋生物学和水产动物病害防控研究

    通讯作者:

    金 珊

  • 中图分类号: Q78; Q959.223.63

Cloning and expression pattern analysis of a lipopolysaccharide -and β-1,3-glucan-binding protein in Portunus trituberculatus

More Information
    Author Bio:

    ZHANG Ying

    Corresponding author: JIN Shan
  • 摘要: 脂多糖-β-1, 3-葡聚糖结合蛋白(LGBP)作为一种模式识别受体在甲壳动物的先天免疫中占有重要地位。利用SMART RACE技术, 从三疣梭子蟹(Portunus trituberculatus) 血细胞中克隆得到一条LGBP基因。该基因cDNA序列全长为1 378 bp, 包括1 095 bp的开放阅读框(ORF), 共编码365个氨基酸, 在基因的5'端还含有138 bp的非编码区(UTR), 3'端含有144 bp的UTR。预测的成熟肽相对分子质量为39 825.24 k, 等电点为4.49。同时, 用生物信息学方法对该基因的序列和二级结构进行了初步分析。RT-PCR结果显示:LGBP基因在被检测的组织, 包括血细胞、肝胰腺、心脏、鳃和肌肉中都有表达。用金黄色葡萄球菌(Staphyloccocus aureus) 和溶藻弧菌(Vibrio alginolyticus) 刺激三疣梭子蟹后, 发现在实验48 h内, 混合细菌刺激组血细胞中LGBP基因的表达量明显高于生理盐水对照组, 推测该基因在抵抗细菌感染的免疫过程中发挥着积极的作用。
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出版历程
  • 收稿日期:  2010-03-09
  • 修回日期:  2010-09-16
  • 刊出日期:  2010-12-22

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