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Isolation and characterization of Hainantoxin-II, a new neurotoxic peptide from the Chinese bird spider (Haplopelma hainanum)

PAN Jian-Yi YU Zhi-Qiang

PAN Jian-Yi, YU Zhi-Qiang. Isolation and characterization of Hainantoxin-II, a new neurotoxic peptide from the Chinese bird spider (Haplopelma hainanum). Zoological Research, 2010, 31(6): 570-574. doi: 10.3724/SP.J.1141.2010.06570
Citation: PAN Jian-Yi, YU Zhi-Qiang. Isolation and characterization of Hainantoxin-II, a new neurotoxic peptide from the Chinese bird spider (Haplopelma hainanum). Zoological Research, 2010, 31(6): 570-574. doi: 10.3724/SP.J.1141.2010.06570

Hainantoxin-Ⅱ的分离纯化及其结构与功能分析(英文)

doi: 10.3724/SP.J.1141.2010.06570
基金项目: 浙江省教育厅科研项目(Y200805989)
详细信息
    作者简介:

    潘建义

    通讯作者:

    潘建义

  • 中图分类号: Q959.226; Q956; Q51

Isolation and characterization of Hainantoxin-II, a new neurotoxic peptide from the Chinese bird spider (Haplopelma hainanum)

Funds: This work was supported by the Research Project of the Education Department of Zhejiang Province, China (Y200805989)
More Information
    Author Bio:

    PAN Jian-Yi

    Corresponding author: PAN Jian-Yi
  • 摘要: 从分布于我国海南省的海南捕鸟蛛 (Haplopelma hainanum) 毒素中, 利用阳离子交换色谱和反相高效液相色谱分离得到一种多肽神经毒素, 命名为Hainantoxin-Ⅱ(HnTx-Ⅱ)。MALDI-TOF质谱分析表明该多肽毒素相对分子质量为4 253.135, 其氨基酸序列经Edman降解测序为LFECS VSCEI EKEGN KDCKK KKCKG GWKCK FNMCV KV, 其中的6个Cys形成3对二硫键。同源性搜索表明, HnTx-Ⅱ与Huwentoxin-Ⅱ(HwTx-Ⅱ)的同源性高达91%, 仅有3个氨基酸残基不同。HwTx-Ⅱ为从虎纹捕鸟蛛中提取的杀虫肽, 该多肽具有独特的结构模体。活性分析表明HnTx-Ⅱ与HwTx-Ⅱ具有相似的生物学活性。经腹腔注射HnTx-Ⅱ, 美洲蜚蠊可被麻痹, 其ED50为16 μg/g; 而当剂量增加到60 μg/g时, 可立即杀死美洲蜚蠊, 其杀死昆虫活性明显强于HwTx-Ⅱ。此外, HnTx-Ⅱ经脑室注射可杀死小鼠, 其LD50为1.41 μg/g, 该活性却明显低于HwTx-Ⅱ。这两种多肽毒素的结构与功能的差异为进一步阐明多肽毒素的结构­与功能之间的关系提供良好的研究模型。
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出版历程
  • 收稿日期:  2010-06-04
  • 修回日期:  2010-10-19
  • 刊出日期:  2010-12-22

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