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Blood transcriptome analysis reveals gene expression features of breast-feeding rhesus macaque (Macaca mulatta) infants

Yue Lan Jiao Wang Qiao Yang Rui-Xiang Tang Min Zhou Guang-Lun Lei Jing Li Liang Zhang Bi-Song Yue Zhen-Xin Fan

Yue Lan, Jiao Wang, Qiao Yang, Rui-Xiang Tang, Min Zhou, Guang-Lun Lei, Jing Li, Liang Zhang, Bi-Song Yue, Zhen-Xin Fan. Blood transcriptome analysis reveals gene expression features of breast-feeding rhesus macaque (Macaca mulatta) infants. Zoological Research, 2020, 41(4): 431-436. doi: 10.24272/j.issn.2095-8137.2020.044
Citation: Yue Lan, Jiao Wang, Qiao Yang, Rui-Xiang Tang, Min Zhou, Guang-Lun Lei, Jing Li, Liang Zhang, Bi-Song Yue, Zhen-Xin Fan. Blood transcriptome analysis reveals gene expression features of breast-feeding rhesus macaque (Macaca mulatta) infants. Zoological Research, 2020, 41(4): 431-436. doi: 10.24272/j.issn.2095-8137.2020.044


doi: 10.24272/j.issn.2095-8137.2020.044

Blood transcriptome analysis reveals gene expression features of breast-feeding rhesus macaque (Macaca mulatta) infants

Funds: This work was supported by the Sichuan Application Foundation Project (2020YJ0303) and Chengdu Giant Panda Breeding Research Foundation (CPF2017-19)
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    Corresponding author: E-mail: zxfan@scu.edu.cn
  • #Authors contributed equally to this work
  • 摘要:

    在母乳喂养期间,婴儿会经历快速生长发育阶段和显著的生理变化。然而,关于母乳喂养期婴儿的基因表达特征和性别特异性基因表达的研究却很少。在这项研究中,我们对16只母乳喂养的猕猴婴猴和他们各自尚在哺乳期的母猴的共32个血液转录组进行了测序分析。我们鉴定到了218个婴猴与母猴之间的差异表达基因(DEG),其中91个DEG在婴猴组中表达上调,127个DEG在婴猴组中表达下调。通过对婴猴的上调DEG和独有的hub基因的功能富集分析,我们发现它们主要分布在免疫、生长和发育等方面。蛋白质互作分析还发现婴猴中的关键基因主要也是与生长发育和免疫相关。此外,我们还在雌雄婴猴之间鉴定到了23个DEG,其中两个DEG位于X染色体,11个位于Y染色体。TMF1监管核内蛋白1 (Trnp1)在哺乳动物中对大脑皮层发育至关重要,该基因在雌性婴猴中显著表达上调。总的来说,我们的研究为非人灵长类哺乳期婴猴的基因表达特征提供了新的见解,并揭示了这些婴猴之间的性别特异性基因表达。

    #Authors contributed equally to this work
  • Figure  1.  Heat map and enrichment analysis of DEGs and weighted gene co-expression network analysis (WGCNA)

    A: Heat map plot of DEGs using gene expression values normalized by Deseq2. Expression values of 32 individuals are presented after being centered and scaled in the row direction. Each column represents a specimen and each row represents a gene. Red indicates up-regulated genes and blue indicates down-regulated genes. B: Bar plot of GO terms of up-regulated DEGs in infants. C: In total, 11 modules were identified by WGCNA. D: Protein-protein interaction network of DEGs and hub genes in infants. Size and gradient color of node are adjusted by degree, and thickness and gradient color of edge are adjusted by combined score. Low values are indicated by dark colors and small sizes. DEGs are circled in red.

    Table  1.   The DEGs between female and male infants

    Gene IDGene nameChromosomeLog2 fold changePadj
    ENSMMUG00000000192MAP7D2chrX2.840 2926.79E–25
    ENSMMUG00000041274ENSMMUG00000041274chrY–22.469 84.23E–05
    ENSMMUG00000046378ZFYchrY–12.224 46.01E–56
    ENSMMUG00000054467ENSMMUG00000054467chrY–7.683 346.10E–10
    ENSMMUG00000042046C14H11orf87chr147.377 8260.002 683
    ENSMMUG00000045991USP9YchrY–13.284 91.72E–69
    ENSMMUG00000038182UTYchrY–12.551 69.93E–62
    ENSMMUG00000059200ENSMMUG00000059200chr136.556 070.004 637
    ENSMMUG00000043966DDX3YchrY–14.122 41.06E–78
    ENSMMUG00000049458ENSMMUG00000049458chrY–9.074 964.46E–26
    ENSMMUG00000060008LY6Dchr84.700 8070.028 138 7
    ENSMMUG00000049951ENSMMUG00000049951chrX9.939 9432.84E–34
    ENSMMUG00000043715EIF1AYchrY–11.388 66.20E–15
    ENSMMUG00000056331PRKXchrX–4.430 092.69E–20
    ENSMMUG00000021031TRNP1chr14.054 1050.022 402 2
    ENSMMUG00000061378ENSMMUG00000061378chrY–7.757 772.89E–12
    ENSMMUG00000046495TBL1YchrY–10.186 42.25E–05
    ENSMMUG00000055339ENSMMUG00000055339chr1626.352 571.62E–08
    ENSMMUG00000038331RPS4Y1chrY–17.108 11.73E–117
    ENSMMUG00000055357ENSMMUG00000055357chr10–21.304 40.000 173 9
    ENSMMUG00000049104CYorf15AchrY–10.877 82.01E–44
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  • 收稿日期:  2020-02-29
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