留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

Non-invasive genetic analysis indicates low population connectivity in vulnerable Chinese gorals: concerns for segregated population management

Jing Yang Guo-Fen Zhu Jian Jiang Chang-Lin Xiang Fu-Li Gao Wei-Dong Bao

Jing Yang, Guo-Fen Zhu, Jian Jiang, Chang-Lin Xiang, Fu-Li Gao, Wei-Dong Bao. Non-invasive genetic analysis indicates low population connectivity in vulnerable Chinese gorals: concerns for segregated population management. Zoological Research, 2019, 40(5): 439-448. doi: 10.24272/j.issn.2095-8137.2019.058
Citation: Jing Yang, Guo-Fen Zhu, Jian Jiang, Chang-Lin Xiang, Fu-Li Gao, Wei-Dong Bao. Non-invasive genetic analysis indicates low population connectivity in vulnerable Chinese gorals: concerns for segregated population management. Zoological Research, 2019, 40(5): 439-448. doi: 10.24272/j.issn.2095-8137.2019.058

非损伤遗传结构分析显示易危物种中华斑羚种群之间的连通性偏低:关注隔离种群的保护

doi: 10.24272/j.issn.2095-8137.2019.058

Non-invasive genetic analysis indicates low population connectivity in vulnerable Chinese gorals: concerns for segregated population management

Funds: was supported by the Beijing Municipal Committee of Science and Technology(Z121100000312107)
More Information
    Corresponding author: Wei-Dong Bao
  • 摘要: 详细了解野生动物种群数量和遗传结构信息,对于制定有效的管理对策至关重要,尤其是对于那些受到人为干扰种群处于下降和隔离状态的珍稀物种更加重要。本文借助非损伤采集粪样、线粒体与核DNA检测等分子标记技术,对来自北京和内蒙古的中华斑羚(Naemorhedus griseus)进行了种群结构比较与监测研究。结果发现,307份粪样被确认为中华斑羚遗留,其中鉴定出北京种群有15只个体(9 雌6雄)、内蒙古种群为61 只个体(37雌24雄)。在这76只个体线粒体DNA中,检测出8种单倍型、13个核苷酸多态性位点、45个微卫星等位基因。北京和内蒙古种群具有显著不同的遗传结构分化特点,但两地种群均趋向于偏雌性的性别比例,预示着较高的繁殖潜力,对于呈现片段化分布的中华斑羚恢复与保护具有重要意义。据此,我们建议对这两个具有丰富遗传多样性的种群作为进化显著单元实施保护,促进现有种群恢复,并考虑在不同种群之间交流个体,以确保中华斑羚种群长期繁盛。
  • [1]
    null An J , Min MS , Sommer J , Louis E Jr , Brenneman R , Kwon SW , Shin NS , Lee H . 2005. Isolation and characterization of 15 microsatellite loci in the Korean goral (Nemorhaedus caudatus). Molecular Ecology Resources, 5(2): 421–423.
    null An J , Choi SK , Sommer J , Louis E Jr , Brenneman R , Zemanova B , Hájková P , Park G , Min MS , Kim KS , Lee H . 2010. A core set of microsatellite markers for conservation genetics studies of Korean goral (Naemorhedus caudatus) and its cross-species amplification in Caprinae species. Journal of Veterinary Science, 11(4): 351–353.
    null Bandelt HJ , Forster P , R?hl A . 1999. Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16(1): 37–48.
    null Banks SC , Lindenmayer DB . 2014. Inbreeding avoidance, patch isolation and matrix permeability influence dispersal and settlement choices by male Agile antechinus in a fragmented landscape. Journal of Animal Ecology, 83(2): 515–524.
    null Bradshaw CJA , Harcourt RG , Davis LS . 2003. Male-biased sex ratios in New Zealand fur seal pups relative to environmental variation. Behavioral Ecology and Sociobiology , 53(5): 297–307.
    null Broquet T , Ménard N , Petit E . 2007. Noninvasive population genetics: a review of sample source, diet, fragment length and microsatellite motif effects on amplification success and genotyping error rates. Conservation Genetics, 8(1): 249–260.
    null Cameron EZ . 2004. Facultative adjustment of mammalian sex ratios in support of the Trivers-Willard hypothesis: evidence for a mechanism. Proceedings of the Royal Society of London B, 271(1549): 1723–1728.
    null Chen W , Gao W , Fu BQ . 2002. Mammals of Beijing. Beijing: Beijing Publishing House. (in Chinese)
    null Chen RB , Liu FY , Tian L , Zhang ZG . 1999. Quantitative distribution of Naemorhedus goral in Daqingshan Mountains. Acta Scientiarum Naturalium Universitatis Neimongol. 30(2): 227–229. (in Chinese)
    null Clark AB . 1978. Sex ratio and local resource competition in a prosimian primate. Science, 201(4351): 163–165.
    null Cockburn A , Legge S , Double MC . 2002. Sex ratios in birds and mammals: can the hypotheses be disentangled?. In: Hardy ICW (ed). Sex Ratios: Concepts and Research Methods. Cambridge: Cambridge University Press, 266–286.
    null DeSalle R , Amato G . 2004. The expansion of conservation genetics. Nature Reviews Genetics, 5: 702–712.
    null Dharmarajan G , Beasley JC , Fike JA , Rhodes OE Jr . 2013. Effects of landscape, demographic and behavioral factors on kin structure: testing ecological predictions in a mesopredator with high dispersal capability. Animal Conservation, 17(3): 225–234.
    null Duckworth JW , MacKinnon J , Tsytsulina K . 2008. Naemorhedus caudatus. The IUCN red list of threatened species 2008: e. T14295A4429742. Retrieved 10 December 2015.
    null Excoffier L , Lischer HEL . 2010. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10(3): 564–567.
    null Fu YX . 1997. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics, 147(2): 915–925.
    null Haag T , Santos AS , De Angelo C , Srbek-Araujo AC , Sana DA , Morato RG , Salzona FM , Eizirik E . 2009. Development and testing of an optimized method for DNA-based identification of jaguar (Panthera onca) and puma (Puma concolor) faecal samples for use in ecological and genetic studies. Genetica, 136(3): 505–512.
    null Hrabina P . 2015. A new insight into the taxonomy and zoogeography of recent species of goral (Nemorhaedus, Bovidae, Ruminantia). Gazella, 42: 33–91.
    null Kalinowski ST , Taper ML , Marshall TC . 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology, 16(5): 1099–1106.
    null Keil P , Storch D , Jetz W . 2015. On the decline of biodiversity due to area loss. Nature Communications, 6: 8837.
    null Kim BJ , Lee H , Lee SD . 2010. Species- and sex-specific multiple PCR amplifications of partial cytochromeb gene and Zfx/Zfy introns from invasive and non-invasive samples of Korean ungulates. Genes & Genomics, 31(5): 369–375.
    null Kim BJ , Lee YS , An J , Park PKC , Okumura H , Lee H , Min MS . 2008. Species and sex identification of the Korean goral (Nemorhaedus caudatus) by molecular analysis of non-invasive samples. Molecules & Cells, 26(3): 314–318.
    null Kruuk LEB , Clutton-Brock TH , Albon SD , Pemberton JM , Guinness FE . 1999. Population density affects sex ratio variation in red deer. Nature, 399: 459–461.
    null Lalitha S . 2000. Primer premier 5. Biotech Software & Internet Report, 1: 270–272.
    null Lan H , Jin K . 2016. Infrared-triggered camera technology application in the investigation of mammals in Beijing Wulingshan national nature reserve. Acta Theriologica Sinica, 36(3): 322–329. (in Chinese)
    null Lande R . 1988. Genetics and demography in biological conservation. Science, 241(4872): 1455–1460.
    null Larkin MA , Blackshields G , Brown NP , Chenna R , McGettigan PA , McWilliam H , Valentin F , Wallace IM , Wilm A , Lopez R , Thompson JD , Gibson TJ , Higgins DG . 2007. Clustal W and Clustal X Version 2.0. Bioinformatics, 23(21): 2947–2948.
    null Lewis S , Benvenuti S , Dall’Antonia L , Griffiths R , Money L , Sherratt TN , Wanless S , Hamer KC . 2002. Sex-specific foraging behaviour in a monomorphic seabird. Proceedings of the Royal Society of London B: Biological Sciences, 269(1501): 1687–1693.
    null Li GL . 2005. Annals of Saihanwula Nature Reserve. Chifeng: Inner Mongolia Science and Technology Press. (in Chinese)
    null Li M , Meng S , Wei F , Wang J , Yong Y . 2003. Genetic diversity and population genetic structure of takin (Budorcas taxicolor). Acta Theriologica Sinica, 23(1): 10–16. (in Chinese)
    null Librado P , Rozas J . 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25(11): 1451–1452.
    null Lindstr?m J , Coulson T , Kruuk L , Forchhammer MC , Coltman DW , Clutton-Brock T . 2002. Sex-ratio variation in Soay sheep. Behavioral Ecology and Sociobiology, 53(1): 25–30.
    null Liu F , Li DQ , Wu JG . 2012. Using infra-red cameras to survey wildlife in Beijing Songshan National Nature Reserve. Acta Ecologica Sinica, 32(3): 730–739. (in Chinese)
    null Liu W , Yao YF , Yu Q , Ni QY , Zhang MW , Yang JD , Mai MM , Xu HL . 2013. Genetic variation and phylogenetic relationship between three serow species of the genus Capricornis based on the complete mitochondrial DNA control region sequences. Molecular Biology Reports, 40(12): 6793–6802.
    null Majumder A , Qureshi Q , Sankar K , Kumar A . 2017. Long-term monitoring of a Bengal tiger (Panthera tigris tigris) population in a human-dominated landscape of Central India. European Journal of Wildlife Research, 63: 17.
    null Mead JI . 1989. Nemorhaedus goral. Mammalian Species, 335: 1–5.
    null Mondol S , Karanth KU , Kumar NS , Gopalaswamy AM , Andheria A , Ramakrishnan U . 2009. Evaluation of non-invasive genetic sampling methods for estimating tiger population size. Biological Conservation, 142(10): 2350–2360.
    null Nei M . 1987. Molecular Evolutionary Genetics. New York: Columbia University Press.
    null Neigel JE , Avise JC . 1993. Application of a random walk model to geographic distributions of animal mitochondrial DNA variation. Genetics, 135(4): 1209–1220.
    null Paetkau D , Waits LP , Clarkson PL , Craighead L , Strobeck C . 1997. An empirical evaluation of genetic distance statistics using microsatellite data from bear (Ursidae) populations. Genetics, 147(4): 1943–1957.
    null Peakall R , Smouse PE . 2010. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28(19): 2537–2539.
    null Piggott MP , Bellemain E , Taberlet P , Taylor AC . 2004. A multiplex pre-amplification method that significantly improves microsatellite amplification and error rates for faecal DNA in limiting conditions. Conservation Genetics, 5(3): 417–420.
    null Scandura M . 2005. Individual sexing and genotyping from blood spots on the snow: a reliable source of DNA for non-invasive genetic surveys. Conservation Genetics, 6(5): 871–874.
    null Slatkin M . 1995. A measure of population subdivision based on microsatellite allele frequencies. Genetics, 139(1): 457–462.
    null Smith AT , Xie Y . 2008. A guide to the mammals of China. New Jersey: Princeton University Press.
    null State Forestry Administration . 2009. Resources survey on national key terrestrial wild animals in China. Beijing: China Forestry Publishing House. (in Chinese)
    null Székely T , Weissing FJ , Komdeur J . 2014. Adult sex ratio variation: implications for breeding system evolution. Journal of Evolutionary Biology, 27(8): 1500–1512.
    null Taberlet P , Griffin S , Goossens B , Questiau S , Manceau V , Escaravage N , Waits LP , Bouvet J . 1996. Reliable genotyping of samples with very low DNA quantities using PCR. Nucleic Acids Research, 24(16): 3189–3194.
    null Taberlet P , Waits LP , Luikart G . 1999. Noninvasive genetic sampling: look before you leap. Trends in Ecology& Evolution, 14(8): 323–327.
    null Tajima F . 1989. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics, 123(3): 585–595.
    null Tamura K , Peterson D , Peterson N , Stecher G , Nei M , Kumar S . 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology & Evolution, 28(10): 2731–2739.
    null Tang SP , Mu LG , Wang XL , Zhang J , Liu B , Menghedalai, Bao WD . 2019. Habitat suitability assessment based on MaxEnt modeling of Chinese goral in Saihanwula national nature reserve, Inner Mongolia of northern China. Journal of Beijing Forestry University, 41(1): 102–108. (in Chinese)
    null Van Oosterhout C , Hutchinson WF , Wills DP , Shipley P . 2004. MICRO‐CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes, 4: 535–538.
    null Waits LP , Luikart G , Taberlet P . 2001. Estimating the probability of identity among genotypes in natural populations: cautions and guidelines. Molecular Ecology, 10(1): 249–256.
    null Wang XM , Cao LR , Liu ZS , Fang SG . 2006. Mitochondrial DNA variation and matrilineal structure in blue sheep populations of Helan Mountain, China. Canadian Journal of Zoology, 84(10): 1431–1439.
    null Xiong Z , Chen M , Zhang E , Huang MJ . 2013. Molecular phylogeny and taxonomic status of the red goral by cyt b gene analyses. Folia Zoologica, 62(2): 125–129.
    null Zhang YS , Jiang WJ , Jiang J , Wang D , Wu JG , Liu FM , Bao WD . 2017. Biodiversity monitoring of undergrowth birds and mammals in Beijing Songshan National Nature Reserve. Chinese Journal of Wildlife, 38(3): 367–375. (in Chinese)
    null Zhang W , Zhang Z , Xu X , Wei K , Wang X , Liang X , Zhang L , Shen FJ , Hou R , Yue BS . 2009. A new method for DNA extraction from feces and hair shafts of the South China tiger (Panthera tigris amoyensis). Zoo Biology, 28(1): 49–58.
  • [1] Xing-Han Chen, Sen Yang, Wei Yang, Yuan-Yuan Si, Rui-Wen Xu, Bin Fan, Le Wang, Zi-Ning Meng.  First genetic assessment of brackish water polychaete Tylorrhynchus heterochaetus: mitochondrial COI sequences reveal strong genetic differentiation and population expansion in samples collected from southeast China and north Vietnam, Zoological Research. doi: 10.24272/j.issn.2095-8137.2020.006
    [2] Jin-Jin MIN, Rong-Hui YE, Gen-Fang ZHANG, Rong-Quan ZHENG.  Microsatellite analysis of genetic diversity and population structure of freshwater mussel (Lamprotula leai), Zoological Research. doi: 10.13918/j.issn.2095-8137.2015.1.34
    [3] Ya HE, Zheng-Huan WANG, Xiao-Ming WANG.  Genetic diversity and population structure of a Sichuan sika deer (Cervus sichuanicus) population in Tiebu Nature Reserve based on microsatellite variation, Zoological Research. doi: 10.13918/j.issn.2095-8137.2014.6.528
    [4] Jingyang HU, Yaping ZHANG, Li YU.  Summary of Laurasiatheria (Mammalia) Phylogeny, Zoological Research. doi: 10.3724/SP.J.1141.2012.E05-06E65
    [5] Kai HE, Naiqing HU, Joseph D. ORKIN, Daw Thida NYEIN, Chi MA, Wen XIAO, Pengfei FAN, Xuelong JIANG.  Molecular phylogeny and divergence time of Trachypithecus: with implications for the taxonomy of T. phayrei, Zoological Research. doi: 10.3724/SP.J.1141.2012.E05-06E104
    [6] ZHENG Wen-Juan, LAI Yu-Hong, YOU Xin-Yu, QIN Xi-Han, ZHU Shi-Hua.  Genetic diversity of Pseudosciaena polyactis in Zhoushan based on mitochondrial DNA D-loop region sequences, Zoological Research. doi: 10.3724/SP.J.1141.2012.03329
    [7] Zhiying JIA, Yuyong ZHANG, Shuqiang CHEN, Lianyu SHI.  Genetic diversity and differentiation of masu salmon (Oncorhynchus masou masou) between and within cultured populations inferred from microsatellite DNA analysis, Zoological Research. doi: 10.3724/SP.J.1141.2012.E03-04E33
    [8] MA Ke-Yi, FENG Jian-Bin, XIE Nan, FENG Xiao-Yu, LI Jia-Le.  Microsatellite analysis of genetic variation of the oriental river prawn Macrobrachium nipponense in Qiantang River, Zoological Research. doi: 10.3724/SP.J.1141.2011.04363
    [9] LIU Bo, KUANG You-Yi, TONG Guang-Xiang, YIN Jia-Sheng.  Analysis of genetic diversity on 9 wild stocks of Taimen (Hucho taimen) by microsatellite markers, Zoological Research. doi: 10.3724/SP.J.1141.2011.06597
    [10] LIN Fang-Jun, JIANG Ping-Ping, DING Ping.  Genetic analysis of microsatellite polymorphism in the Elliot’s Pheasant (Syrmaticus ellioti) in China, Zoological Research. doi: 10.3724/SP.J.1141.2010.05461
    [11] ZHONG Ping-hua, SHAO Ming-qin, DAI Nian-hua, ZENG Fan-wei.  Genetic Differentiation of Tegillarca granosa Based on Mitochondrial COI Gene Sequences, Zoological Research. doi: 10.3724/SP.J.1141.2009.01017
    [12] SHEN Yu-bang, LI Jia-le, FENG Bing-bing.  Genetic Analysis of Cultured and Wild Populations of Mytilus coruscus Based on Mitochondrial DNA, Zoological Research. doi: 10.3724/SP.J.1141.2009.03240
    [13] QI De-lin.  Genetic Variation and Diversity of Gymncypris eckloni in the Upper Yellow River Inferred from Mitochondrial Cytochrome b Gene, Zoological Research. doi: 10.3724/SP.J.1141.2009.03255
    [14] LIU Qing-shen, FENG Ding-yuan, ZHANG Hui-ming, LIU Shen-fu.  Genetic Diversity of Chinese Shar-pei Dog Using Microsatellite DNA Markers, Zoological Research.
    [15] YANG Bo, CHEN Xiao-yong, YANG Jun-xing.  Structure of the Mitochondrial DNA Control Region and Population Genetic Diversity Analysis of Anabarilius grahami (Regan), Zoological Research. doi: 10.3724/SP.J.1141.2008.04379
    [16] YE Lang-hui HUO Jin-long MIAO Yong-wang , * ZHU Sheng-quan CHEN Tao LIU Li-xian PAN Wei-rong BI Bao-liang.  Genetic Diversity Analysis of Nixi Chicken Using Microsatellite DNA Markers, Zoological Research.
    [17] ZHOU Li, LIU Jin-Xia, GUI Jian-Fang.  Preliminary Investigation on Genetic Diversity of Gynogenetic Silver Crucian Carp (Carassius auratus gibelio Bloch) Detected by Microsatellite DNA, Zoological Research.
    [18] ZHANG Sheng-qiao, XU Ji-chu, ZHANG Ya-ping, FU Yan, CHEN Yong-jiu.  Genetic Diversity of Zhejiang Native Pig Breeds Inferred From Mitochondrial DNA Polymorphism, Zoological Research.
    [19] LI Xiang-long, ZHANG Ya-ping, CHEN Shen-gou, ZENG Fan-tong, QIU Xiang-pin, LIU Xiang-mo.  Study on The mtDNA Rflp of Goat Breeds, Zoological Research.
    [20] ZHANG Ya-ping.  A Non-Invasive Approach to The Genetic Analysis of Asiatic Black Bear, Zoological Research.
  • 加载中
计量
  • 文章访问数:  574
  • HTML全文浏览量:  68
  • PDF下载量:  182
  • 被引次数: 0
出版历程
  • 收稿日期:  2019-03-12
  • 刊出日期:  2019-09-18

目录

    /

    返回文章
    返回