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Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder)

Chun-Lei WANG Zhi-Peng WANG Jia-Qi WANG Ming-You Li Xiao-Wu CHEN

Chun-Lei WANG, Zhi-Peng WANG, Jia-Qi WANG, Ming-You Li, Xiao-Wu CHEN. Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder). Zoological Research, 2016, 37(5): 301-306. doi: 10.13918/j.issn.2095-8137.2016.5.301
Citation: Chun-Lei WANG, Zhi-Peng WANG, Jia-Qi WANG, Ming-You Li, Xiao-Wu CHEN. Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder). Zoological Research, 2016, 37(5): 301-306. doi: 10.13918/j.issn.2095-8137.2016.5.301

牙鲆性腺候选piRNAs的鉴定

doi: 10.13918/j.issn.2095-8137.2016.5.301
基金项目: This research was supported by grants from the China Agriculture Research System (CARS-49), National Natural Science Foundation of China (31372520) and Shanghai Collaborate Innovation Center for Aquatic Animal Genetics and Breeding (ZF1206)
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    通讯作者:

    Xiao-Wu CHEN

Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder)

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    Corresponding author: Xiao-Wu CHEN
  • 摘要: 在硬骨鱼类性腺的发育和功能维持中,与Piwi蛋白发生互作的RNA(piRNA)发挥重要作用。本研究采用二代测序技术获得牙鲆(Paralichthys olivaceus)成熟性腺的piRNA文库。以斑马鱼piRNA作为参考获得5865个特候选piRNA分子,最后组成289个piRNA簇(PRCs)。在分离出来的候选piRNAs分子中,有53.42%和28.42%分别在精巢和卵巢中优先表达。有38个PRCs在卵巢中特异表达,45个PRCs在卵巢中高表达,24个PRCs在精巢中特异表达的,131个PRCs在精巢中高表达;通过定量PCR反应验证七个PRCs的相对表达水平,和测序结果一致。本论文将有助于探讨牙鲆的发育和性别维持的分子机理。
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  • 收稿日期:  2016-04-08
  • 修回日期:  2016-09-05
  • 刊出日期:  2016-09-18

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