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Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream (Megalobrama amblycephala)

Shou-Jie TANG Si-Fa LI Wan-Qi CAI Yan ZHAO

Shou-Jie TANG, Si-Fa LI, Wan-Qi CAI, Yan ZHAO. Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream (Megalobrama amblycephala). Zoological Research, 2014, 35(2): 108-117. doi: 10.11813/j.issn.0254-5853.2014.2.108
Citation: Shou-Jie TANG, Si-Fa LI, Wan-Qi CAI, Yan ZHAO. Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream (Megalobrama amblycephala). Zoological Research, 2014, 35(2): 108-117. doi: 10.11813/j.issn.0254-5853.2014.2.108

Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream (Megalobrama amblycephala)

doi: 10.11813/j.issn.0254-5853.2014.2.108
基金项目: This work was supported by the National Natural Science Foundation of China (30630051) and Doctoral Research Initial Funding from Shanghai Ocean University (A-2400-11-0186).
详细信息
    通讯作者:

    Si-Fa LI

Microsatellite analysis of variation among wild, domesticated, and genetically improved populations of blunt snout bream (Megalobrama amblycephala)

  • 摘要: In the present study, the genetic diversity of one selected strain (Pujiang No. 1), two domesticated populations (GA and HX) and four wild populations (LZ, YN, SS and JL) of blunt snout bream (Megalobrama amblycephala) was analyzed using 17 microsatellite markers. The results showed that an average of 4.88-7.65 number of alleles (A); an average of 3.20-5.33 effective alleles (Ne); average observed heterozygosity (Ho) of 0.6985-0.9044; average expected heterozygosity (He) of 0.6501-0.7805; and the average polymorphism information content (PIC) at 0.5706-0.7226. Pairwise FST value between populations ranged from 0.0307-0.1451, and Nei's standard genetic distance between populations was 0.0938-0.4524. The expected heterozygosities in the domesticated populations (GA and HX) were significantly lower than those found in three wild populations (LZ, SS and JL), but no difference was detected when compared with the wild YN population. Likewise, no difference was found between the four wild populations or two domesticated populations. The expected heterozygosity in Pujiang No. 1 was higher than the two domesticated populations and lower than the four wild populations. Regarding pairwise FST value between populations, permutation test P-values were significant between the GA, HX and PJ populations, but not between the four wild populations. These results showed that the expected heterozygosity in the selected strain of blunt snout bream, after seven generations of selective breeding, was lower than that of wild populations, but this strain retains higher levels of genetic diversity than domesticated populations. The genetic differences and differentiation amongst wild populations, domesticated populations and the genetically improved strain of blunt snout bream will provide important conservation criteria and guide the utilization of germplasm resources.
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  • 收稿日期:  2013-09-18
  • 修回日期:  2013-12-11
  • 刊出日期:  2014-03-08

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