Abstract: Using the method of electrophresis,the lactate dehydrogenase (LDH) and population relationship of Yunnan and Guangxi tree shrews is studied by isozyme analysis.There are diverged in their tissues and also in different animals inhabit in different place,but no electrophoretic difference observed between malcs and females for the analysis of LDH isozyme in the six tissues (heart,liver,lung,spleen,kidney and muscle).The results suggested that these tree shrews are three subspecies which confirm some reports on morphology tudy in tree shrews.