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Genetic diversity of Pseudosciaena polyactis in Zhoushan based on mitochondrial DNA D-loop region sequences

ZHENG Wen-Juan LAI Yu-Hong YOU Xin-Yu QIN Xi-Han ZHU Shi-Hua

ZHENG Wen-Juan, LAI Yu-Hong, YOU Xin-Yu, QIN Xi-Han, ZHU Shi-Hua. Genetic diversity of Pseudosciaena polyactis in Zhoushan based on mitochondrial DNA D-loop region sequences. Zoological Research, 2012, 33(3): 329-336. doi: 10.3724/SP.J.1141.2012.03329
Citation: ZHENG Wen-Juan, LAI Yu-Hong, YOU Xin-Yu, QIN Xi-Han, ZHU Shi-Hua. Genetic diversity of Pseudosciaena polyactis in Zhoushan based on mitochondrial DNA D-loop region sequences. Zoological Research, 2012, 33(3): 329-336. doi: 10.3724/SP.J.1141.2012.03329

舟山小黄鱼线粒体DNA D-loop区序列变异的遗传多样性分析

doi: 10.3724/SP.J.1141.2012.03329
详细信息
  • 中图分类号: Q349;Q953.3;Q959.483

Genetic diversity of Pseudosciaena polyactis in Zhoushan based on mitochondrial DNA D-loop region sequences

  • 摘要: 小黄鱼(Pseudosciaena polyactis)为我国重要海产经济鱼类之一, 过度捕捞和环境污染等因素造成其资源日益衰退。研究小黄鱼种群遗传结构对其资源的保护及其可持续利用有十分重要的意义。该研究采用聚合酶链式反应(PCR)技术对浙江舟山附近海域小黄鱼种群53个个体的mtDNA D-loop区全序列进行扩增, 序列长度在795~801bp 之间, 长度差异不大。采用Clustal X1.83、MEGA3.1、DnaSP4.0等生物信息学软件进行遗传多样性分析, 结果显示:53条小黄鱼线粒体DNA D-loop 区的T、C、A 和G 碱基平均含量分别为30.3%、23.1%、32.3%和14.3%, 排除13 处核苷酸的插入或缺失后, 共检测到93处转换和颠换位点, 约占分析序列总长度的11.6%, 其中包括53 个单一多态位点和40 个简约信息位点, 共确定了52 种单倍型, 单倍型多样性(hd)为0.9993, 单倍型间的平均遗传距离为0.012, 转换/颠换平均值为4.305, 平均核苷酸差异数(k)为9.73875, 核苷酸多样性(π)为0.01233,表明舟山小黄鱼遗传多样性处于中等水平。
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  • 收稿日期:  2011-11-15
  • 修回日期:  2012-02-21
  • 刊出日期:  2012-06-22

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