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Measuring the estrus cycle and its effect on superovulation in mice

ZHU Na JIA Hong-Xiang LIU Xiao-Kun ZHAO Xiao-E WEI Qiang MA Bao-Hua

ZHU Na, JIA Hong-Xiang, LIU Xiao-Kun, ZHAO Xiao-E, WEI Qiang, MA Bao-Hua. Measuring the estrus cycle and its effect on superovulation in mice. Zoological Research, 2012, 33(3): 276-282. doi: 10.3724/SP.J.1141.2012.03276
Citation: ZHU Na, JIA Hong-Xiang, LIU Xiao-Kun, ZHAO Xiao-E, WEI Qiang, MA Bao-Hua. Measuring the estrus cycle and its effect on superovulation in mice. Zoological Research, 2012, 33(3): 276-282. doi: 10.3724/SP.J.1141.2012.03276

小鼠发情周期卵泡发育动态及其对超数排卵的影响

doi: 10.3724/SP.J.1141.2012.03276
基金项目: 中国科学院战略性先导科技专项(XDA04020222-03)
详细信息
  • 中图分类号: Q492; Q954.431; Q959.837

Measuring the estrus cycle and its effect on superovulation in mice

  • 摘要: 该文探讨了小鼠发情周期中阴门状态、阴道脱落细胞类型变化规律、卵泡发育规律及其相互关系, 并比较了发情周期不同阶段的超排效果。结果表明, 采用阴门状态观察法和阴道脱落细胞涂片法, 能有效判断小鼠发情周期阶段。卵巢组织切片观察结果表明, 在发情周期不同阶段, 小鼠的卵泡发育和黄体的生成与消退存在明显的规律性变化; 小鼠发情周期中, 其阴门状态、阴道脱落细胞种类及卵泡发育动态之间存在相关关系; 发情周期不同阶段开始超排的小鼠, 其配种见栓率和回收胚胎平均数均存在明显差异, 发情前期显著优于发情后期与间情期(P<0.05), 并高于发情期, 但差异不显著(P>0.05),即阴门状态观察法与阴道脱落细胞涂片法均可用于小鼠发情周期阶段的判断, 发情前期为最适宜的小鼠超排时期。
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出版历程
  • 收稿日期:  2012-02-24
  • 修回日期:  2012-03-17
  • 刊出日期:  2012-06-22

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