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Molecular cloning, sequence analysis and expression of ayu complement component C9 gene

KONG Cheng-Jiang HUANG Zuo-An CHEN Jiong SHI Yu-Hong LU Xin-Jiang

KONG Cheng-Jiang, HUANG Zuo-An, CHEN Jiong, SHI Yu-Hong, LU Xin-Jiang. Molecular cloning, sequence analysis and expression of ayu complement component C9 gene. Zoological Research, 2012, (2): 151-157. doi: 10.3724/SP.J.1141.2012.02151
Citation: KONG Cheng-Jiang, HUANG Zuo-An, CHEN Jiong, SHI Yu-Hong, LU Xin-Jiang. Molecular cloning, sequence analysis and expression of ayu complement component C9 gene. Zoological Research, 2012, (2): 151-157. doi: 10.3724/SP.J.1141.2012.02151

香鱼补体成分C9基因的克隆、序列分析及表达

doi: 10.3724/SP.J.1141.2012.02151
基金项目: 教育部新世纪优秀人才支持计划项目(NCET-08-0928); 宁波市自然科学基金项目(2010A610001)
详细信息
  • 中图分类号: Q785; Q959.499; Q516

Molecular cloning, sequence analysis and expression of ayu complement component C9 gene

  • 摘要: 补体成分C9是构成膜攻击复合体引起靶细胞溶解破坏的重要组成成分。该文测定了香鱼C9(aC9)基因的cDNA全序列,序列全长2125个核苷酸,编码一个由592个氨基酸组成、相对分子质量为6.56×104的前体蛋白,N端22个氨基酸为信号肽序列。序列分析表明,aC9与虹鳟C9的氨基酸同源性最高,达56.8%,与其它鱼类C9的同源性介于40.9%~53.8%之间。aC9在健康香鱼肝、脾、肠、鳃和肌肉有表达,其中在肝内的表达量最高。实时荧光定量PCR的结果显示,鳗利斯顿氏菌侵染4h后,肝中aC9mRNA表达量显著上调,并随着时间的推移在16h时达到峰值。Western blotting分析的结果显示,鳗利斯顿氏菌侵染后香鱼血清中的aC9蛋白随着时间的推移呈显著上调。以上结果表明,香鱼肝组织C9基因表达变化与鳗利斯顿氏菌的侵染密切相关,揭示了C9在鱼类抗细菌免疫反应中具有重要的作用。
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出版历程
  • 收稿日期:  2011-11-21
  • 修回日期:  2011-12-26
  • 刊出日期:  2012-04-22

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