残锷线蛱蝶线粒体基因组全序列及其系统学意义

TIAN Li-Li; SUN Xiao-Yan; CHEN Mei; GAI Yong-Hua; HAO Jia-Sheng; YANG Qun

doi:10.3724/SP.J.1141.2012.02133

残锷线蛱蝶线粒体基因组全序列及其系统学意义

doi: 10.3724/SP.J.1141.2012.02133

1.
安徽师范大学生命科学学院分子进化与生物多样性研究室安徽芜湖 241000;
2.
中国科学院南京地质古生物研究所现代古生物学与地层学国家重点实验室南京 210008

基金项目: This work was supported by the National Natural Science Foundation of China (41172004), the CAS/SAFEA International Partnership Program for Creative Research Teams, Chinese Academy of Sciences (KZCX22YW2JC104), the Provincial Key Projectof the Natural Science Foundation from the Anhui Province, China (KJ2010A142), and the Open Funds from the State KeyLaboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences

详细信息

中图分类号: Q969.42; Q969.439.2; Q754
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- 收稿日期: 2011-11-18
- 修回日期: 2012-02-28
- 刊出日期: 2012-04-22

Complete mitochondrial genome of the Five-dot Sergeant Parathyma sulpitia (Nymphalidae: Limenitidinae) and its phylogenetic implications

1.
College of Life Sciences, Anhui Normal University, Wuhu 241000, China;
2.
LPS, Institute of Geology and Palaeontology,the Chinese Academy of Sciences, Nanjing 210008, China

Funds: This work was supported by the National Natural Science Foundation of China (41172004), the CAS/SAFEA International Partnership Program for Creative Research Teams, Chinese Academy of Sciences (KZCX22YW2JC104), the Provincial Key Projectof the Natural Science Foundation from the Anhui Province, China (KJ2010A142), and the Open Funds from the State KeyLaboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences

摘要

摘要: 对残锷线蛱蝶(Parathyma sulpitia)(鳞翅目:蛱蝶科)线粒体基因组全序列进行了测定。结果表明:残锷线蛱蝶线粒体基因组全序列全长为15268bp,除了在trnS1(AGN)和trnE基因之间有一段121bp长的基因间隔外,其基因的排列顺序及排列方向与大多数已测鳞翅目物种基本一致。在蛋白质编码基因中,除cox1以CGA作为其起始密码子之外,其余12个蛋白质编码基因都以标准的ATN作为起始密码子。此外,除nad4基因以单独的T为终止密码子,其余12个蛋白质编码基因都以TAA结尾。除trnS1(AGN)缺少DHU臂之外,22个tRNA基因都显示典型的三叶草形二级结构。除A+T富集区外的非编码序列中,线粒体基因组共含有11个基因间隔区。其中,最长的一个121bp的基因间隔区位于trnS1(AGN)和trnE之间,其A+T含量高达100%。另外,和其他鳞翅目物种一样,在其A+T富集区的3’端有一段长达18bp的poly-T结构。A+T富集区内部没有明显的小卫星样多拷贝重复序列,而含有一些微卫星样的重复结构。本研究基于13种蛋白编码基因序列的组合数据,用最大似然法和贝叶斯法对蛱蝶科几个主要亚科间共9个代表物种间的系统发生关系进行了分析。结果表明,本研究的结果与前人的分子系统学研究结论基本吻合(其中,线蛱蝶亚科和釉蛱蝶亚科互为姐妹群),而与形态学的研究结论不一致。
- 残锷线蛱蝶 /
- 鳞翅目 /
- 蛱蝶科 /
- 线蛱蝶亚科 /
- 线粒体基因组
Abstract: The complete mitochondrial genome of the Parathyma sulpitia (Lepidoptera, Nymphalidae, Limenitidinae) was determined. The entire mitochondrial DNA (mtDNA) molecule was 15 268 bp in size. Its gene content and organization were the same as those of other lepidopteran species, except for the presence of the 121 bp long intergenic spacer between trnS1(AGN) and trnE. The 13 protein-coding genes (PCGs) started with the typical ATN codon, with the exception of the cox1 gene that used CGA as its initial codon. In addition, all protein-coding genes terminated at the common stop codon TAA, except the nad4 gene which used a single T as its terminating codon. All 22 tRNA genes possessed the typical clover leaf secondary structure except for trnS1(AGN), which had a simple loop with the absence of the DHU stem. Excluding the A+T-rich region, the mtDNA genome of P. sulpitia harbored 11 intergenic spacers, the longest of which was 121 bp long with the highest A+T content (100%), located between trnS1(AGN) and trnE. As in other lepidopteran species, there was an 18-bp poly-T stretch at the 3’-end of the A+T-rich region, and there were a few short microsatellite-like repeat regions without conspicuous macro-repeats in the A+T-rich region. The phylogenetic analyses of the published complete mt genomes from nine Nymphalidae species were conducted using the concatenated sequences of 13 PCGs with maximum likelihood and Bayesian inference methods. The results indicated that Limenitidinae was a sister to the Heliconiinae among the main Nymphalidae lineages in this study, strongly supporting the results of previous molecular data, while contradicting speculations based on morphological characters.
- Parathyma sulpitia /
- Lepidoptera /
- Nymphalidae /
- Limenitidinae /
- Mitochondrial genome

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残锷线蛱蝶线粒体基因组全序列及其系统学意义

doi: 10.3724/SP.J.1141.2012.02133

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Complete mitochondrial genome of the Five-dot Sergeant Parathyma sulpitia (Nymphalidae: Limenitidinae) and its phylogenetic implications

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残锷线蛱蝶线粒体基因组全序列及其系统学意义

doi: 10.3724/SP.J.1141.2012.02133

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Complete mitochondrial genome of the Five-dot Sergeant Parathyma sulpitia (Nymphalidae: Limenitidinae) and its phylogenetic implications

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