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畅 悦, 冯立芳, 熊 杰, 缪 炜. 2011: 嗜热四膜虫金属硫蛋白基因MMT2MTT4的表达规律比较与进化模式分析. 动物学研究, 32(5): 476-484. DOI: 10.3724/SP.J.1141.2011.05476
引用本文: 畅 悦, 冯立芳, 熊 杰, 缪 炜. 2011: 嗜热四膜虫金属硫蛋白基因MMT2MTT4的表达规律比较与进化模式分析. 动物学研究, 32(5): 476-484. DOI: 10.3724/SP.J.1141.2011.05476
CHANG Yue, FENG Li-Fang, XIONG Jie, MIAO Wei. 2011: Function comparison and evolution analysis of metallothionein gene MTT2 and MTT4 in Tetrahymena thermophila. Zoological Research, 32(5): 476-484. DOI: 10.3724/SP.J.1141.2011.05476
Citation: CHANG Yue, FENG Li-Fang, XIONG Jie, MIAO Wei. 2011: Function comparison and evolution analysis of metallothionein gene MTT2 and MTT4 in Tetrahymena thermophila. Zoological Research, 32(5): 476-484. DOI: 10.3724/SP.J.1141.2011.05476

嗜热四膜虫金属硫蛋白基因MMT2MTT4的表达规律比较与进化模式分析

Function comparison and evolution analysis of metallothionein gene MTT2 and MTT4 in Tetrahymena thermophila

  • 摘要: 多个金属硫蛋白基因异构型已在四膜虫中被鉴定, 这些异构型可分为7a和7b两个亚家族。该文利用实时荧光定量PCR技术检测了嗜热四膜虫金属硫蛋白基因MTT2和MTT4在Hg、Cu、Cd、Zn、H2O2暴露下的表达水平, 结果显示两者表达规律相似, 均为:Cu暴露下上调最高(>200倍), Hg次之, Cd、Zn上调倍数不大, H2O2有下调趋势。此表达规律明显有别于7a亚家族, 具有7b亚家族的表达特征。同种诱导物暴露下MTT4的上调表达幅度比MTT2高出数倍, 结合生物信息学分析结果, 推测可能与MTT2和MTT4上游调控元件(如AP-1、MRE等)的数量差异有关。基于MTT2和MTT4在结构和功能上的高度相似性, 推测两者可能是经近期基因复制事件产生, 并遵循基因剂量模型进化而来。

     

    Abstract: Tetrahymena has a high genetic polymorphism of metallothionein proteins. These protein isoforms can be divided into subfamilies 7a and 7b. We used real-time quantitative PCR to test the expression levels of Tetrahymena thermophila metallothionein genes MTT2 and MTT4 after exposure to different inducers, including Hg, Cu, Cd, Zn and H2O2. Both genes were most efficiently induced by Cu and secondly by Hg. Their expression was slightly up-regulated after exposure to Cd and Zn, but down-regulated by H2O2. The expression pattern differed from those reported in the 7a subfamily, but was consistent with 7b subfamily members. However, the induced expression level of MTT4 was much higher than that of MTT2, which might be due to differences in their upstream regulatory elements (AP-1, MRE). The high similarities in gene structures and functions indicate that MTT2 and MTT4 were generated by recent gene duplication following the dosage balance model.

     

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