悬尾应激对小鼠空间记忆及其反转学习的损伤效应
Impairment effects of tail suspension stress on spatial memory and its reversal learning in mice
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摘要: 该文探讨了连续数天短时悬尾应激对小鼠十字迷宫空间记忆及其反转学习的作用。81只成年雄性昆明小鼠被分为4大组:绝对空间记忆获得组及巩固组; 相对空间记忆获得组及巩固组。每大组又分为悬尾组(每天训练前或训练后立即接受悬尾处理20 min)和对照组。结果表明, 在空间记忆训练初期, 各对照组和悬尾组动物正确反应率无明显差异, 均在机遇水平; 随着训练天数的增加, 对照组成绩显著提高, 当其正确反应率达到80%时, 悬尾组正确反应率仍处于或略高于机遇水平, 两组间差异显著(P < 0.01); 在反转学习中, 悬尾组正确反应率也显著低于对照组(P < 0.01)。这些表明,悬尾应激可显著损伤小鼠的空间记忆及其反转学习的获得和巩固, 其中相对空间记忆及其反转学习的巩固受损尤为严重。Abstract: Present work investigated the effects of tail suspension stress (TSS) on spatial memory acquisition, consolidation, and its reversal learning in mice. Eighty-one adult male KM mice were divided into four groups (each group including a TSS subgroup and its control subgroup): absolute spatial memory acquisition and consolidation groups (group AA and CA); relative spatial memory acquisition and consolidation groups (group AR and CR). TSS (20 min) was performed immediately before (acquisition) or after (consolidation) a daily training. Results showed that there was no significant difference between control animals and TSS animals in each group in early spatial memory training days (5-8 d of training). Along with training, the performance of control animals improved significantly, but the performance of TSS animals improved slightly (group AA, CA and AR) or even did not change (group CR) (P < 0.01). Reversal learning was also impaired in TSS animals (P < 0.01). The results indicated that TSS could impair spatial memory acquisition, consolidation and reversal learning (especially the relative spatial memory consolidation and its reversal learning) in mice.