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A traumatic brain injury model for distinguishing between transplanted neural cells and host cells in vivo

YANG Shang-Chuan DONG Jin-Run QU Jia-Gui HU Xin-Tian WANG Zheng-Bo

YANG Shang-Chuan, DONG Jin-Run, QU Jia-Gui, HU Xin-Tian, WANG Zheng-Bo. A traumatic brain injury model for distinguishing between transplanted neural cells and host cells in vivo. Zoological Research, 2011, 32(4): 421-427. doi: 10.3724/SP.J.1141.2011.04421
Citation: YANG Shang-Chuan, DONG Jin-Run, QU Jia-Gui, HU Xin-Tian, WANG Zheng-Bo. A traumatic brain injury model for distinguishing between transplanted neural cells and host cells in vivo. Zoological Research, 2011, 32(4): 421-427. doi: 10.3724/SP.J.1141.2011.04421

一种有效区分移植细胞和宿主细胞脑损伤模型的建立

doi: 10.3724/SP.J.1141.2011.04421
基金项目: “973”项目(2007CB947703)
详细信息
    作者简介:

    杨上川, E-mail: Yangsc@mail.kiz.ac.cn

    通讯作者:

    胡新天,王正波

A traumatic brain injury model for distinguishing between transplanted neural cells and host cells in vivo

Funds: Mechanical damaging method; Neural cell; Host cell; Neural stem cells; Traumatic brain injury
More Information
    Author Bio:

    YANG Shang-Chuan,E-mail: Yangsc@mail.kiz.ac.cn

    Corresponding author: HU Xin-Tian,WANG Zheng-Bo
  • 摘要: 为了区分移植神经细胞和宿主细胞, 便于将来在宿主体内对移植细胞进行在体的电生理记录以及其它方面的研究, 通过机械损毁的方法, 建立了一种特殊的脑损伤模型。结果发现, 通过机械损毁的方法, 在大鼠大脑皮层形成形态规则的损伤空洞, 其模型稳定, 重复性好; 在空洞内进行干细胞移植, 能够长时间存活, 移植神经干细胞绝大部分细胞分化为神经元, 只有少量细胞分化为胶质细胞, 而且移植细胞与宿主细胞分界明显; 对移植细胞进行单细胞电生理记录, 记录到神经元放电信号。这些结果表明, 通过机械损毁的方法, 在大鼠大脑皮层成功建立了一个稳定、精确定位移植细胞与宿主细胞界限的脑损伤模型。
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出版历程
  • 收稿日期:  2011-01-06
  • 修回日期:  2011-04-14
  • 刊出日期:  2011-08-22

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