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Notch signaling dependent differentiation of cholangiocyte-likecells from rhesus monkey embryonic stem cells

JIN Li-Fang JI Shao-Hui YANG Ji-Feng JI Wei-Zhi

JIN Li-Fang, JI Shao-Hui, YANG Ji-Feng, JI Wei-Zhi. Notch signaling dependent differentiation of cholangiocyte-likecells from rhesus monkey embryonic stem cells. Zoological Research, 2011, 32(4): 391-395. doi: 10.3724/SP.J.1141.2011.04391
Citation: JIN Li-Fang, JI Shao-Hui, YANG Ji-Feng, JI Wei-Zhi. Notch signaling dependent differentiation of cholangiocyte-likecells from rhesus monkey embryonic stem cells. Zoological Research, 2011, 32(4): 391-395. doi: 10.3724/SP.J.1141.2011.04391

Notch 信号通路调控猕猴胚胎干细胞的胆管样细胞分化(英文)

doi: 10.3724/SP.J.1141.2011.04391
详细信息
    作者简介:

    金立方(1978-),男,浙江上虞人,讲师,博士,研究方向为干细胞生物学;E-mail: lifangj@sohu.com

    通讯作者:

    季维智

Notch signaling dependent differentiation of cholangiocyte-likecells from rhesus monkey embryonic stem cells

Funds: This work was supported by research grants from Zhejiang Natural Sciences Foundation of China (Y2110911; Y2080996), and the National Key Technologies R&D Program of China (2007CB947701)
More Information
    Author Bio:

    JIN Li-Fang,lifangj@sohu.com

    Corresponding author: JI Wei-Zhi
  • 摘要: 猕猴胚胎干细胞(rhesus monkey embryonic stem (rES))与人胚胎干细胞有相似的生物学特性, 因此是理想的临床前研究的替代模型。Notch信号通路在胆管及胆管上皮细胞的形成中有重要的作用, 然而, 有关Notch信号通路在ES细胞的胆向分化中的作用了解甚少。该实验以rES为模型, 对Notch信号通路对ES细胞的胆向分化过程中的作用进行了较为系统的研究。rES在细胞因子Activin A诱导作用下产生约80%的限定性内胚层细胞。以Matrigel作为细胞外基质, 在含BMP4和FGF1的无血清培养体系中继续诱导5~7 d, rES细胞来源的限定性内胚层细胞分化产生约胆管样细胞。分化的细胞表达胆管细胞的特异性蛋白((CK7、CK18、CK19、CK20 和OV-6)及基因(GSTPi、IB4 和 HNF1β)。在胆管样细胞的分化过程中检测到了Notch 1和Notch 2基因及下游信号分子hes 1和hes 5的表达。用Notch抑制剂L-685458处理分化过程中的细胞可导致Notch 1和Notch 2基因及下游信号分子hes 1和hes 5的表达下降, 同时CK19阳性的胆管样细胞分化比率也从90%下降至约20%。这一研究提示Notch信号通路可能在ES细胞的胆管样分化过程中扮演重要的角色。
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出版历程
  • 收稿日期:  2011-01-21
  • 修回日期:  2011-06-11
  • 刊出日期:  2011-08-22

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