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Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis

QI Zhi-Tao ZHANG Qi-Huan WANG Zi-Sheng WANG Ai-Min HUANG Bei CHANG Ming-Xian

QI Zhi-Tao, ZHANG Qi-Huan, WANG Zi-Sheng, WANG Ai-Min, HUANG Bei, CHANG Ming-Xian. Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis. Zoological Research, 2011, 32(4): 371-378. doi: 10.3724/SP.J.1141.2011.04371
Citation: QI Zhi-Tao, ZHANG Qi-Huan, WANG Zi-Sheng, WANG Ai-Min, HUANG Bei, CHANG Ming-Xian. Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis. Zoological Research, 2011, 32(4): 371-378. doi: 10.3724/SP.J.1141.2011.04371

非洲爪蟾中一种长型肽聚糖识别蛋白的克隆与表达分析(英文)

doi: 10.3724/SP.J.1141.2011.04371
详细信息
    作者简介:

    齐志涛

    通讯作者:

    齐志涛

Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis

Funds: This study was financially supported by the Project from the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (10KJB240001), the Foundation for Talent Recruitment of Yancheng Institute of Technology (XKR2011007), and the National Natural Science Foundation of China (30830083)
More Information
    Author Bio:

    QI Zhi-Tao1

    Corresponding author: QI Zhi-Tao
  • 摘要: 肽聚糖识别蛋白(peptidoglycan recognition proteins, PGRPs)是固有免疫系统中一类重要的模式识别受体。该文首次从两栖类模式生物-非洲爪蟾 (Xenopus tropicalis)中克隆得到了一个长型PGRP(XtPGRP-L)基因。XtPGRP-L具有5个外显子和4个内含子的基因组结构, 该结构在进化的过程中比较保守。序列比对与系统进化分析显示XtPGRP-L具有保守的酰胺酶活性位点。蛋白质建模显示XtPGRP-L拥有保守的3-D结构。实时定量PCR检测显示, XtPGRP-L在非洲爪蟾胚胎早期不表达, 到72 h蝌蚪期开始表达。在成体的肝脏、肺、肠和胃高表达。同时, 在LPS刺激后, XtPGRP-L在肝脏、肠和胃中呈明显上调表达。结果表明,XtPGRP-L在非洲爪蟾固有免疫系统中可能具有重要的作用
  • [1]  Arnold K, Bordoli L, Kopp J, Schwede T. 2006. The SWISS-MODEL workspace: a web-based environment for protein structure homology modeling [J]. Bioinformatics, 22: 195-201.
    [2]  Chang MX, Nie P, Wei LL. 2007. Short and long peptidoglycan recognition proteins (PGRPs) in zebrafish, with findings of multiple PGRP homologs in teleost fish [J]. Mol Immunol, 44: 3005-3023.
    [3]  Chang MX, Nie P. 2008. RNAi suppression of zebrafish peptidoglycan recognition protien 6 (zfPGRP6) mediated differentially expressed genes involved in Toll-like receptor signaling pathway and caused increased susceptibility to Flavobacterium columnare [J]. Vet Immunol Immunopathol, 124: 295-301.
    [4]  Chang MX, Wang YP, Nie P. 2009. Zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways
    [J]. Fish Shellfish Immunol, 26: 264-274.
    [6]  Cheng X, Zhang X, Pflugrath JW, Studier FW. 1994. The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase [J]. Proc Natl Acad Sci USA, 91: 4034-4038.
    [7]  Dale L, Slack JM. 1987. Fate map for the 32-cell stage of Xenopus laevis [J]. Development, 99: 527-551.
    [8]  Dimopoulos G, Christophides GK, Meister S, Schultz J, White KP, Barillas-Mury C, Kafatos FC. 2002. Genome expression analysis of Anopheles gambiae: Responses to injury, bacterial challenge, and malaria infection [J]. Proc Natl Acad Sci USA, 99: 8814-8819.
    [9]  Dziarski R. 2004. Peptidoglycan recognition proteins (PGRPs) [J]. Mol Immunol, 40: 877-886.
    [10]  Jones DT. 1999. Protein secondary structure prediction based on position-specific scoring matrices [J]. J Mol Biol, 292:195-202.
    [11]  Li XN, Wang SY, Qi J, Echtenkamp SF, Chatterjee R, Wang M, Boons GJ, Dziarski R, Gupta D. 2007. Zebrafish peptidoglycan recognition proteins are bactericidal amidases essential for defense against bacterial infections [J]. Immunity, 27: 518–529.
    [12]  Liang SC, Tan XY, Luxenberg DP, Karim R, Dunussi-Joannopoulos K, Collins M, Fouser LA. 2006. Interleukin 22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides [J]. J Exp Med, 203: 2271–2279.
    [13]  Liu C, Gelius E, Liu G, Steiner H, ziarski R. 2000. Mammalian peptidoglycan recognition protein binds peptidoglycan with high affinity, is expressed in neutrophils, and inhibits bacterial growth [J]. J Biol Chem, 275: 24490-24499.
    [14]  Liu C, Xu Z, Gupta D, Dziarski R. 2001. Peptidoglycan recognition proteins: A novel family of four human innate immunity pattern recognition molecules [J]. J Biol Chem, 276: 34686–34694.
    [15]  McGuffin L, Bryson K, Jones DT. 2000. The PSIPRED protein structure prediction server [J]. Bioinformatics, 16: 404-405.
    [16]  Purcell MK, Kurath G, Garver KA, Herwig RP, Winton JR. 2004. Quantitative expression profiling of immune response genes in rainbow trout following infectious haematopoietic necrosis virus (IHNV) infection or DNA vaccination [J]. Fish Shellfish Immunol, 17: 262-447.
    [17]  Qi ZT, Nie P. 2008. Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis [J]. Immunogenetics, 60:699-710.
    [18]  Qi ZT, Gao Q, Huang B, Chang MX, Nie P. 2010. Cloning and identification of a short type Peptidoglycan recognition protein in Xenopus tropicalis [J]. Acta Hyrobiol Sin, 34:922-926. (in Chinese)
    [19]  Reiser JB, Teyton L, Wilson IA. 2004. Crystal structure of the Drosophila peptidoglycan recognition protein (PGRP)-SA at 1.56 A resolution [J]. J Mol Biol, 340: 909-917.
    [20]  Schwede T, Kopp J, Guex N, Peitsch MC. 2003. SWISS-MODEL: An automated protein homology-modeling server [J]. Nucleic Acids Res, 31: 3381-3385.
    [21]  Steiner H. 2004. Peptidoglycan recognition proteins: on and off switches for innate immunity [J]. Immunol Rev, 198: 83-96.
    [22]  Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice [J]. Nucleic Acids Res, 22: 4673-4680.
    [23]  Wang YP, Lai R. 2010. Insect antimicrobial peptides: structures, properties and gene regulation [J]. Zool Res, 31: 27-34. (in Chinese)
    [24]  Wang ZM, Li X, Cocklin RR, Wang M, Fukase K, Inamura S, Kusumoto S, Gupta D, Dziarski R. 2003. Human peptidoglycan recognition protein-L is an N-acetylmuramoyl-L-alanine amidase [J]. J Biol Chem, 278(49): 49044-49052.
    [25]  Werner T, Liu G, Kang D, Ekengren S, Steiner H, Hultmark D. 2000. A family of peptidoglycan recognition proteins in the fruit fly Drosophila melanogaster [J]. Proc Natl Acad Sci USA, 97: 13772-13777.
    [26]  Yoshida H, Kinoshita K, Ashida M. 1996. Purification of a peptidoglycan recognition protein from hemolymph of the silkworm, Bombyx mori [J]. J Biol Chem, 271: 13854-13860.
    [27]  
    [28]  
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出版历程
  • 收稿日期:  2011-03-15
  • 修回日期:  2011-06-27
  • 刊出日期:  2011-08-22

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