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Microsatellite analysis of genetic variation of the oriental river prawn Macrobrachium nipponense in Qiantang River

MA Ke-Yi FENG Jian-Bin XIE Nan FENG Xiao-Yu LI Jia-Le

MA Ke-Yi, FENG Jian-Bin, XIE Nan, FENG Xiao-Yu, LI Jia-Le. Microsatellite analysis of genetic variation of the oriental river prawn Macrobrachium nipponense in Qiantang River. Zoological Research, 2011, 32(4): 363-370. doi: 10.3724/SP.J.1141.2011.04363
Citation: MA Ke-Yi, FENG Jian-Bin, XIE Nan, FENG Xiao-Yu, LI Jia-Le. Microsatellite analysis of genetic variation of the oriental river prawn Macrobrachium nipponense in Qiantang River. Zoological Research, 2011, 32(4): 363-370. doi: 10.3724/SP.J.1141.2011.04363

钱塘江日本沼虾野生群体遗传变异的SSR分析

doi: 10.3724/SP.J.1141.2011.04363
基金项目: 国家自然科学基金项目(31001111); 上海高校选拔培养优秀青年教师科研专项基金项目(SSC08004); 浙江省杭州市科技局种子种苗项目(20071032H27); 上海海洋大学博士科研启动基金项目(B-8201-08-0279)
详细信息
    作者简介:

    第一作者简介:马克异, 男, 硕士研究生, E-mail: mky850125@163.com

    通讯作者:

    李家乐

Microsatellite analysis of genetic variation of the oriental river prawn Macrobrachium nipponense in Qiantang River

More Information
    Author Bio:

    MA Ke-Yi,E-mail: mky850125@163.com

    Corresponding author: LI Jia-Le
  • 摘要: 利用微卫星标记分析了钱塘江干流水域闻堰、富阳、场口、桐庐等7个野生日本沼虾群体的遗传多样性和遗传结构。结果表明:10个微卫星位点呈现高度多态性; 闻堰、富阳、场口、新安江等中下游野生群体的遗传多样性水平有高于歙县和休宁两个上游群体遗传多样性水平的趋势。符号检验和Wilcoxon符号秩次检验的结果表明, 钱塘江日本沼虾群体近期没有发生瓶颈效应, 群体数量也没有下降。FST的范围介于0.0201 ~ 0.1069。分子方差分析结果显示, 大部分的遗传变异(93.48%)存在于个体间, 少部分遗传变异(6.52%)存在于群体之间。群体间FST及AMOVA分析表明, 群体处于中等遗传分化水平; 基于DA遗传距离构建的NJ聚类树显示, 地理位置相邻的群体聚在一起。STRUCTURE分析413份参试的日本沼虾样本被分为2个理论种群, 即上游歙县和休宁群体为一个理想种群, 其余中下游的5个群体为另一个理想种群。日本沼虾的遗传多样性和遗传结构与所生存的地理位置具有相关性。
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出版历程
  • 收稿日期:  2010-11-08
  • 修回日期:  2011-05-02
  • 刊出日期:  2011-08-22

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