郑氏比蜢线粒体基因组全序列的测定与分析

杨辉; 黄原

doi:10.3724/SP.J.1141.2011.04353

郑氏比蜢线粒体基因组全序列的测定与分析

杨辉,
黄原

Analysis of the complete mitochondrial genome sequence of Pielomastax zhengi

摘要

摘要: 采用长距PCR扩增及保守引物步移法测定并注释了郑氏比蜢(Pielomastaxzhengi)的线粒体基因组全序列。郑氏比蜢的线粒体基因组全长15602bp,A+T含量为71.8%,37个基因位置与飞蝗的一致,基因间隔序列共计10处47bp,间隔长度从1～20bp不等;有14对基因间存在52bp重叠,重叠碱基数在1～8bp之间。蛋白质基因的起始密码子均为昆虫典型的起始密码子ATN。ND5基因使用了不完全终止密码子T,其余基因均为典型的TAA或TAG。除tRNASer(AGN)的DHU臂缺失外,其余21个tRNA基因的二级结构均属典型的三叶草结构,但在郑氏比蜢中有5个tRNA(tRNACys、tRNALys、tRNAPhe、tRNAProtRNAArg)基因变异较大,无法采用常规算法预测出来,表现在这5个tRNA二级结构的TψC臂仅有3～4对配对碱基,tRNALys和tRNAArg的反密码臂仅有4对配对碱基。预测的lrRNA二级结构总共有6个结构域(结构域Ⅲ缺失),44个茎环结构。预测的srRNA的二级结构包含3个结构域,30个茎环结构。比较郑氏比蜢、西藏飞蝗(Locustamigratoriatibetensis)和疑钩额螽(Ruspoliadubia)rRNA二级结构后，发现郑氏比蜢与西藏飞蝗的更相似。A+T丰富区中存在一个被认为与复制及转录起始有关的Ploy(T)结构。

Abstract: ThecompletemitochondrialgenomesequenceofPielomastaxzhengiwasdeterminedbyusinglongPCRandconservedprimerwalkingapproaches.TheresultsshowedthattheentiremitochondrialgenomeofPielomastaxzhengiis15602bplongwithA+Tcontentof71.8%.All37genesareconservedinthepositionsobservedinthoseofLocustamigratoria.Allthegenesarecloselyassembledbyleaving10intergenicspacersinbetween.Thoseintergenicspacersare47bpintotal(excludingtheA+Trichregion),withindividualsizerangesfrom1bpto20bp.Inaddition,thereareatotalof52bpoverlappingsequencesamong14genes,rangingfrom1−8bp.Allprotein-codinggenesstartwithatypicalinitiationcodonininsects,ATN.Twelveprotein-codinggenesusetheusualTAAandTAGterminationcodons,whereas,theND5geneshaveanincompleteterminationcodon(T).ExceptthetRNASer(AGN),whoseDHUarmisabsent;alltheother21tRNAgeneshavetypicalclover-leafsecondarystructures.ButinPielomastaxzhengi,thesecondarystructuresoffivetRNA(tRNACys,tRNALys,tRNAPhe,tRNAPro,tRNAArg)genescannotbepredictedbytheconventionalmethodsastheyhaveonly3−4,ratherthan5basepairsintheTψCarm,while,thetRNALysandtRNAArghaveonly4basepairsintheanticodearm.ThepredictedsecondarystructuresoflrRNAandsrRNAhave6domainswith44helicesand3domainswith30helices,respectively.TheresultsoftherRNAsecondarystructurecomparisonshowedthatPielomastaxzhengiismorecloselyrelatedwithLocustamigratoriatibetensisthanRuspoliadubia.Likemost&

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