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Estrogen receptor expression and vitellogenin synthesis induced in hepatocytes of male frogs Rana chensinensis exposed to bisphenol A

BAI Yao ZHANG Yu-Hui ZHAI Li-Li LI Xin-Yi YANG Jun HONG Yan-Yan

BAI Yao, ZHANG Yu-Hui, ZHAI Li-Li, LI Xin-Yi, YANG Jun, HONG Yan-Yan. Estrogen receptor expression and vitellogenin synthesis induced in hepatocytes of male frogs Rana chensinensis exposed to bisphenol A. Zoological Research, 2011, 32(3): 317-322. doi: 10.3724/SP.J.1141.2011.03317
Citation: BAI Yao, ZHANG Yu-Hui, ZHAI Li-Li, LI Xin-Yi, YANG Jun, HONG Yan-Yan. Estrogen receptor expression and vitellogenin synthesis induced in hepatocytes of male frogs Rana chensinensis exposed to bisphenol A. Zoological Research, 2011, 32(3): 317-322. doi: 10.3724/SP.J.1141.2011.03317

双酚A诱导雄性中国林蛙肝细胞雌激素受体表达和卵黄蛋白原合成

doi: 10.3724/SP.J.1141.2011.03317
基金项目: 国家自然科学基金资助项目(30770243)
详细信息
    作者简介:

    白 瑶

    通讯作者:

    张育辉

  • 中图分类号: X174; Q959.53

Estrogen receptor expression and vitellogenin synthesis induced in hepatocytes of male frogs Rana chensinensis exposed to bisphenol A

More Information
    Author Bio:

    BAI Yao

    Corresponding author: ZHANG Yu-Hui
  • 摘要: 为探讨双酚A(bisphenol A, BPA)对雄性两栖动物肝细胞中雌激素受体(estrogen receptor, ER)表达和卵黄蛋白原(vitellogenin, VTG)合成的影响, 将中国林蛙(Rana chensinensis)雄性成体分别持续暴露于10-7、10-6、10-5 mol/L BPA水体中10、20、30 d, 设10-9、10-8 mol/L雌二醇(E2)为阳性对照。用原位杂交技术检测ER mRNA在肝细胞中的表达定位,用免疫组织化学技术检测肝细胞中ER和VTG蛋白的表达。结果显示, 各BPA和E2处理组肝细胞中均有ER mRNA阳性反应, ER和VTG蛋白的表达相对值均显著高于空白对照组。在同一暴露时间, ER和VTG表达值是随着BPA浓度的增加呈增高趋势。在同一BPA浓度, VTG表达值是随暴露时间的延长呈增高趋势, 而ER表达值则无显著性变化。这些表明BPA可以通过诱导雄性中国林蛙肝细胞的ER高调导致VTG合成, 但其效应远低于E2
  • [1] Andrew CD, Marcus E, Taisen I, Susan J, Hans L, Gerald A, Le Blanc, Louis J, Guillette J. 2007. An ecological assessment of bisphenol A: Evidence from comparative biology[J]. Reprod Toxicol, 24: 225-239.Bowman CJ, Kroll KJ, Gross TG, Denslow ND.2002. Estradiol-induced gene expression in largemouth bass (Micropterus salmoides) [J]. Mol Cell Endocrin, 196(1-2): 67-77.Bogi C, Schwaiger J, Ferling H, Mallow U, Steineck C, Sinowatz F, Kalbfus W, Negele RD, Lutz I, Kloas W. 2003. Endocrine effects of environmental pollution on Xenopus laevis and Rana temporaria [J]. Environ Res, 93: 195-201.Denslow ND, Chow MC, Kroll KJ, Green L. 1999. Vitellogenin as a bio-marker of exposure for estrogen or estrogenic mimics [J]. Ecotoxicology, 8(5): 385-398.Denslow ND, Bowman CJ, Ferguson RJ, Lee HS, Hemmer MJ, Folmar LC. 2001. Induction of gene expression in sheeps head minnows (Cyprinodon variegatus) treated with 17β-estradiol, diethylstilbetrol, or ethinylestradiol: the use of mRNA fingerprints as an indicator of gene regulation [J]. Gen Comp Endocrinol, 121(3): 250-260.Flouriot G, Pakdel F, Ducouret B, Ledrean Y, Valotaire Y. 1997. Differential regulation of two genes implicated in fish reproduction: vitellogenin and estrogen receptor genes
    [J]. Mol Reprod Dev, 48(3): 317-323.Gye MC, Kim DH. 2005. Bisphenol A induces hepatic vitellogenin mRNA in male Bombina orientalis [J]. Bull Environ Contam Toxicol, 75: 1-6.Iwamuro S, Sskakibara M, Terao M, Ozawa A, Kurobre C, Shigeura T, Kato M, Kikuyama S. 2003. Teratogenic and anti-metamorphic effects of bisphenol A on embryonic and larval Xenopus laevis [J]. Gen Comp Endocrinol, 133(2): 189-198.Klinge CM. 2001. Estrogen receptor interaction with estrogen response elements [J]. Nucl Acids Res, 29(14): 2905-2919.Kloas W, Einspanier R, Lutz I. 1999. Amphibians as a model to study endocrine disrupters: II. estrogenic activity of environmental chemicals in vitro and in vivo
    [J]. Sci Total Environ, 225(1-2): 59-68.Kloas W, Schrag B, Ehnes C, Segner H. 2000. Binding of xenobiotics to hepatic estrogen receptor and plasma sex steroid binding protein in the teleost fish, the carp (Cyprinus carpio)[J]. Gen Comp Endocrinol, 119: 287-299.Krishnan AV, Stathis P, Permuth SF, Tokes L, Feldman D.1993. Bisphenol A:an estrogenic substance is released from polycarbonate flasks during autoclaving
    [J].Endocrinology, 132: 2279-2286.Levy G, Lutz I, Krüger A, Kloas W. 2004. Bisphenol A induces feminization in Xenopus laevis tadpoles[J]. Environ Res, 94(1): 102-111.Lindholst C, Wynne P M, Marriott P, Pedersen S N, Bjerregaard P.2003. Metabolism of bisphenol A in zebrafish (Danio rerio) and rainbow trout (Oncorhynchus Mykiss) in relation to estrogenic response [J]. Comp Biochem Physiol C: Toxicol Pharmacol, 135: 169-177.Matthews JB, Twomey K, Zacharewski TR. 2001. In vitro and in vivo interactions of bisphenol A and its metabolite, bisphenol A glucuronide with estrogen receptors alpha and beta[J]. Chem Res Toxicol, 14: 149-157.Mitsui N, Tooi O, Kawahara A. 2007.Vitellogenin-inducing activities of natural, synthetic, and environmental estrogens in primary cultured Xenopus laevis hepatocytes
    [J]. Comp Biochem Physiol C: Toxicol Pharmacol, 146:581–587.Pakel F, Féon S, Gac FL, Menn FL, Valotaire Y. 1991. In vivo estrogen induction of hepatic estrogen receptor mRNA and correlation with vitellogenin mRNA in rainbow trout
    [J]. Mol Cell Endocrinol, 75: 205-212. Park CB, Kim BH, Na OS, Choi YC, Lee YD, Baek HJ, Kim HB, Takemura A. 2003. Induction of in vitro vitellogenin synthesis by bisphenol A, nonylphenol and octylphenol in Chinese minnow (Phoxinus oxycephalus) hepatocytes [J]. Korean J Biol Sci, 7: 227-235.Rouhani RT, Sanderson JT, Van HI, Van KP, Bosveld AT, Vanden BM. 2005. Effects of environmental and natural estrogens on vitellogenin production in hepatocytes of the brown frog (Rana temporaria) [J]. Aquat Toxicol, 71: 97-101.Seo JS, Lee YM, Jung SO, Kim IC, Yoon YD, Lee JS. 2006. Nonylphenol modulates expression of androgen receptor and estrogen receptor genes differently in gender types of the hermaphroditic fish Rivulus marmoratus [J]. Biochem Biophys Res Commun, 346(1): 213-223. Sohoni P, Tyler CR, Hurd K, Caunter J, Hetheridge M, Williams T, Woods C, Evans M, Toy R, Gargas M, Sumpter JP. 2001. Reproductive effects of long-term exposure to bisphenol A in the fathead minnow (Pimephales promelas) [J]. Environ Sci Technol, 35 (14): 2917-2925.Takai Y, Tsutsumi O, Ikezuki Y, Hiroi H, Osuga Y, Momoeda M, Yano T, Taketani Y. 2000. Estrogen receptor-mediated effects of a xenoestrogen, bisphenol A, on preimplantation mouse embryos [J]. Biochem Biophys Res Commun, 270(3): 918-921.Takao T, Nanamiya W, Nazarloo HP, Matsumoto R, Asaba K, Hashimoto K. 2003. Exposure to the environmental estrogen bisphenol A differentially modulated estrogen receptor-α and-β immunoreactivity and mRNA in male mouse testis [J]. Life Sci, 72(10): 1159-1169.Welshons WV, Thayer KA, Judy BM, Taylor JA, Curran EM, Vom Saal FS. 2003. Large effects from small exposures. I. Mechanisms for endocrine-disrupting chemicals with estrogenic activity [J]. Environ Health Perspect, 111(8): 994-1006.Yamaguchi A, Ishibashi H, Kohra S, Arizono K, Tominaga N. 2005. Short-term effects of endocrine-disrupting chemicals on the expression of estrogen-responsive genes in male medaka (Oryzias latipes) [J]. Aquat Toxicol, 72(3): 239-249.
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出版历程
  • 收稿日期:  2010-06-29
  • 修回日期:  2011-04-02
  • 刊出日期:  2011-06-22

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