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Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus)

CHEN Wen-Bo WANG Xin ZHOU Ya-Lu DONG Hai-Yan LIN Hao-Ran LI Wen-Sheng

CHEN Wen-Bo, WANG Xin, ZHOU Ya-Lu, DONG Hai-Yan, LIN Hao-Ran, LI Wen-Sheng. Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus). Zoological Research, 2011, 32(3): 285-292. doi: 10.3724/SP.J.1141.2011.03285
Citation: CHEN Wen-Bo, WANG Xin, ZHOU Ya-Lu, DONG Hai-Yan, LIN Hao-Ran, LI Wen-Sheng. Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus). Zoological Research, 2011, 32(3): 285-292. doi: 10.3724/SP.J.1141.2011.03285

尼罗罗非鱼Orexin前体基因的克隆、组织分布及其在摄食调控中的表达

doi: 10.3724/SP.J.1141.2011.03285
基金项目: 现代农业产业技术体系建设专项资金项目(CARS-49); 2007年公益性行业(农业)科研专项经费项目(3-49-10);“863”专题项目(2007AA10Z165)
详细信息
    作者简介:

    陈文波(1982—), 男, 博士, 讲师, 从事鱼类生理学和分子生物学研究, E-mail:chenwenbo@hpu.edu.cn; 王鑫(1987—), 男, 硕士研究生

    陈文波(1982—), 男, 博士, 讲师, 从事鱼类生理学和分子生物学研究, E-mail:chenwenbo@hpu.edu.cn; 王鑫(1987—), 男, 硕士研究生

    通讯作者:

    李文笙(1967—), 女, 汉族, 博士, 教授, 主要从事鱼类生理和分子生物学、神经内分泌学研究。E-mail:lsslws@mail.sysu.edu.cn

  • 中图分类号: Q78; Q959.483

Molecular cloning, tissue distribution and the expression in the regulation of food intake of prepro-orexin in Nile tilapia (Oreochromis niloticus)

More Information
    Author Bio:

    CHEN Wen-Bo,WANG Xin

    CHEN Wen-Bo,WANG Xin

    Corresponding author: LI Wen-Sheng
  • 摘要: 该文采用RT-PCR和cDNA末端快速扩增技术(rapid-amplification of cDNA ends, RACE)的方法, 从尼罗罗非鱼(Oreochromis niloticus)下丘脑总RNA中获得了尼罗罗非鱼Orexin前体基因的cDNA全长序列。该 cDNA全长648 bp, 其中开放阅读框的长423 bp,编码Orexin前体蛋白为140个氨基酸, 包括37个氨基酸的信号肽、43个氨基酸的Orexin-A、28个氨基酸的Orexin-B和末尾32个氨基酸组成的功能不详的多肽。采用Real-time PCR 技术对尼罗罗非鱼Orexin前体基因的组织表达模式以及在摄食前后、饥饿和再投喂状态下的表达量变化进行了研究。结果显示, 在脑部和外周等18个组织中都检测到了Orexin前体基因的表达, 其中在下丘脑中表达量最高; 在摄食前后, 尼罗罗非鱼Orexin前体基因的表达量显著低于在摄食状态中; 饥饿2、4、6和8 d后, Orexin前体基因在下丘脑中的表达量与正常投喂组相比均显著升高, 饥饿4 d再投喂后,表达量又恢复至正常水平。这些结果表明, Orexin在尼罗罗非鱼摄食中可能有着重要的调节作用。
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出版历程
  • 收稿日期:  2010-09-13
  • 修回日期:  2011-02-21
  • 刊出日期:  2011-06-22

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