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Effects of salinity and betaine on BHMT mRNA expression in Lateolabrax japonicus

QIAN Yun-Xia SONG Juan-Juan

QIAN Yun-Xia, SONG Juan-Juan. Effects of salinity and betaine on BHMT mRNA expression in Lateolabrax japonicus. Zoological Research, 2011, 32(3): 277-284. doi: 10.3724/SP.J.1141.2011.03277
Citation: QIAN Yun-Xia, SONG Juan-Juan. Effects of salinity and betaine on BHMT mRNA expression in Lateolabrax japonicus. Zoological Research, 2011, 32(3): 277-284. doi: 10.3724/SP.J.1141.2011.03277

盐度和甜菜碱对鲈鱼BHMT mRNA表达的影响

doi: 10.3724/SP.J.1141.2011.03277
基金项目: 国家自然科学基金(30671608); 浙江省自然科学基金( M303345); 宁波市自然科学基金(2006A610088).
详细信息
    作者简介:

    钱云霞(1965— ), 女, 副教授, 硕士生导师, 主要从事水产动物生物化学与分子生物学研究,Email:qianyunxia@nbu.edu.cn

    通讯作者:

    钱云霞(1965— ), 女, 副教授, 硕士生导师, 主要从事水产动物生物化学与分子生物学研究

  • 中图分类号: Q785; Q959.499; Q516

Effects of salinity and betaine on BHMT mRNA expression in Lateolabrax japonicus

More Information
    Author Bio:

    QIAN Yun-Xia(1965-),email:qianyunxia@nbu.edu.cn

    Corresponding author: QIAN Yun-Xia(1965-)
  • 摘要: 甜菜碱高半胱氨酸甲基转移酶(BHMT, EC 2.1.1.5)催化甜菜碱的甲基转移给高半胱氨酸(Hcy), 而分别生成二甲基甘氨酸和蛋氨酸。利用RT-PCR和SMART RACE的方法从鲈鱼(Lateolabrax japonicus)肝脏中克隆了BHMT全长cDNA。该序列全长1 461 bp, 5'端非翻译区72 bp, 3'端非翻译区183 bp, 开放阅读框1 206 bp, 可编码一个由401个氨基酸组成的蛋白质, 该蛋白质相对分子质量为44.32 kD, 等电点为7.21。氨基酸序列分析表明, BHMT具有较高的保守性, 鲈鱼BHMT与人、小鼠等 9个物种的同源性为77%~93%, 其中与黄鲈(Perca flavescens)同源性最高, 为93%。用RT-PCR分析BHMT基因在10个组织中的表达结果表明, 只有在肝、肠和肾中有较高的表达。RT-PCR和定量PCR表明, 鲈鱼从盐度25的海水转入盐度12的海水后, 肝、肠和肾BHMT基因表达量有增加, 而将鲈鱼从盐度为25的海水转入盐度为29的海水后, 肝、肠和肾的BHMT基因表达则减少。腹腔注射甜菜碱可增加鲈鱼BHMT基因在肝、肠和肾三个组织中的相对表达量。这些结果表明, 甜菜碱可诱导鲈鱼BHMT 基因表达, 而BHMT在适应鱼类环境渗透压变化中起重要作用。
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出版历程
  • 收稿日期:  2010-08-26
  • 修回日期:  2010-11-22
  • 刊出日期:  2011-06-22

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