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张春雷, 佟广香, 匡友谊, 张超, 尹家胜. 2010: 哲罗鱼微卫星亲子鉴定的应用. 动物学研究, 31(4): 395-400. DOI: 10.3724/SP.J.1141.2010.04395
引用本文: 张春雷, 佟广香, 匡友谊, 张超, 尹家胜. 2010: 哲罗鱼微卫星亲子鉴定的应用. 动物学研究, 31(4): 395-400. DOI: 10.3724/SP.J.1141.2010.04395
ZHANG Chun-Lei, TONG Guang-Xiang, KUANG You-Yi, ZHANG Chao, YIN Jia Sheng. 2010: Applicability of Microsatellite DNA Markers to the Parental Identification of Hucho taimen (Pallas). Zoological Research, 31(4): 395-400. DOI: 10.3724/SP.J.1141.2010.04395
Citation: ZHANG Chun-Lei, TONG Guang-Xiang, KUANG You-Yi, ZHANG Chao, YIN Jia Sheng. 2010: Applicability of Microsatellite DNA Markers to the Parental Identification of Hucho taimen (Pallas). Zoological Research, 31(4): 395-400. DOI: 10.3724/SP.J.1141.2010.04395

哲罗鱼微卫星亲子鉴定的应用

Applicability of Microsatellite DNA Markers to the Parental Identification of Hucho taimen (Pallas)

  • 摘要: 该文通过7对微卫星标记,对哲罗鱼的微卫星亲子鉴定准确率、亲子鉴定能力与候选亲本群体大小的关系及应用作了研究。通过计算机软件分析,亲子鉴定能力随候选亲本群体的增大而减小。在81个可能的父母对条件下,单独养殖家系鉴定准确率为80%,混合养殖家系有78.9%的后裔可以鉴定其父母;而在9个可能的父母对条件下,单独养殖家系鉴定准确率为93.3%,混合养殖家系鉴定率为92.2%。该研究表明微卫星分子标记可以用于哲罗鱼的家系鉴定。

     

    Abstract: Using seven pairs of microsatellite markers we studied the relationship between the size of the the candidate groups and the microsatellite paternity testing accuracy by separate breed or the microsatellite paternity appraisal ability by mixed breed. By computer software analysis, the appraisal ability decreased with the candidate groups increase. For the 81 putative parents the microsatellite paternity testing accuracy was 80% and the microsatellite paternity appraisal ability was 78.9%; for the 9 putative parents, the testing accuracy was 93.3% and the appraisal ability was 92.2%. The results indicated that the microsatellite DNA markers can be used for the parentage determination of Hucho taimen Pallas.

     

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