中华鲟及6种淡水养殖鱼类脂蛋白脂酶和肝脂酶基因克隆及系统进化分析
Molecular Characterization and Evolutional Analysis of Liportein Lipase (LPL) and Hepatic Lipase (HL) Gene
in Chinese sturgeon and Other Six Freshwater Fishes
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摘要: 为了研究鱼类脂蛋白脂酶(liportein lipase, LPL)、肝脂酶(hepatic lipase, HL)基因结构、功能及分子系统关系，我们克隆了中华鲟(Acipenser sinensis)、鲢(Hypophthalmichthys molitrix)、鳙(Aristichthys nobilis)、草鱼(Ctenopharyngodon idellus)、鲮鱼(Cirrhinus molitorella)、尼罗罗非鱼(Oreochromis niloticus)和斑鳢(Channa maculata) LPL和HL基因cDNA核心序列，并推测了其相应氨基酸序列。并应用5'RACE和3'RACE技术分别扩增中华鲟、鲢肝脏LPL基因与中华鲟肝脏HL基因cDNA全序列。序列同源性分析表明，LPL和HL氨基酸序列分别在哺乳类动物、鸟类、鱼类中相对保守。与已知的脊椎动物内皮脂酶(endothelial lipase, EL)和胰脂酶(pancreatic lipase, PL)氨基酸序列构建系统进化树，发现LPL、HL、EL和PL同属脂肪酶家族，四者聚集成一有根树。Abstract: In order to study the structural, functional and molecular evolutional relationship of fish liportein lipase (LPL) and hepatic lipase (HL) genes, seven liver LPL and HL cDNA partial sequences were isolated from Acipenser sinensis, Hypophthalmichthys molitrix, Aristichthys nobilis, Ctenopharyngodon idellus, Cirrhinus molitorella, Oreochromis niloticus, Channa maculate by RT-PCR. Three full-length cDNA sequences of LPL, HL of Acipenser sinensis and LPL of Hypophthalmichthys molitrix were obtained by RACEs. From the sequence analysis and homologous results, the amino acid sequences of LPL and HL are relatively conserved in mammals, birds and fishes. Taken together with these obtained amino acid sequences and sequences of all known LPL, HL, EL and PL from other vertebrates, a phylogenetic tree was constructed by neighbor-joining method. The result supports that all of them belong to lipase family.
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