Inhibition Effect of IL-1β on Calcium Channels Currents in Cultured Cortical Neurons of Rat
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摘要: 白细胞介素1β（IL-1β）是重要的促炎细胞因子，在中枢神经系统（CNS）中发挥广泛的生物学功能。在病理条件下，细胞膜上电压门控钙通道的变化与疾病发展过程密切相关。虽然IL-1β和钙通道都在脑损伤和脑疾病过程中发挥重要作用，但目前还很少有两者之间相互关系的研究报道。该研究使用了培养的大鼠胎鼠皮层神经元和膜片钳记录技术，研究了长时间的IL-1β处理对电压门控钙通道电流的作用。结果表明，IL-1β在10和50 ng/mL剂量下都可以抑制钙电流，这种抑制作用具有时间和剂量依赖性的模式，并且不改变钙通道的激活性质。Abstract: Interleukin-1β(IL-1β) is an important porinflammatory cytokine that exert wide biological functions in the central nervous system (CNS). Voltage-gated Ca2+ channels in plasma membrane are essential components of Ca2+ signal whose change on pathological conditions is closely related to processes of diseases. Although both IL-1βand Ca2+ channels have key roles in injury and disease of the brain, so far their relations have been reported in few studies. The effects of IL-1βon Ca2+ channels in cultured cortical neurons of rats were investigated using patch-clamp recording in present research. Our results showed that both 10 and 50 ng/mL of IL-1βtreatment inhibit the Ca2+ currents in time and dose dependent manner, without the change of activation properties of voltage-gated Ca2+ channels (VGCC)s.
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Key words:
- Interleukin-1&beta /
- Ca2+ currents /
- Patch-clamp recording
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