摘要:
测定了4种文蛤属贝类的15个个体的COI基因序列，并从GenBank下载了短文蛤 (M. petechialis)的相应序列。比对后的序列长度为574 bp，包括93个简约信息位点，A、T、C和G的平均含量分别为21.15%、44.71%、14.05%和20.09%。通过对序列的分析，共定义了12个单倍型：文蛤 (M. meretrix) 4个，斧文蛤 (M. lamarckii) 2个，丽文蛤 (M. lusoria) 3个，琴文蛤 (M. lyrata) 1个，短文蛤2个。以青蛤(Cylina sinensis)为外群，用MP法和贝叶斯法构建系统树。结果显示，短文蛤、丽文蛤和文蛤为亲缘关系较近的物种, 支持短文蛤和丽文蛤为文蛤的同物异名的观点。
关键词:
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文蛤属 /
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文蛤 /
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COI基因 /
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系统发育
Abstract:
Fifteen sequences from the mitochondrial cytochrome c oxidase subunit I gene (COI) were determined for 4 species of the genus Meretrix, with the homologous sequences of M. petechialis obtained from the GenBank data library. The alignment length of the sequences was 574 bp after excluding ambiguous sites, including 93 parsimony informative sites. In the fragments, the percentages of A, T, C and G were 21.15%, 44.71%, 14.05% and 20.09% respectively. There were 12 haplotypes identified: 4 M. meretrix, 2 M. lamarckii, 3 M. lusoria, 1 M. lyrata and 2 M. petechialis. Furthermore, it was revealed that M. meretrix, M. petechialis and M. lusoria shared some haplotypes. Phylogeny trees were reconstructed by Maximum-parsimony (MP) and Bayesian method using Cylina sinensis as the outgroup. Our results indicated that M. lusoria, M. petechialis and M. meretrix are closely related species. This is in accordance with the viewpoint that M. petechialis and M. lusoria should be treated as a junior synonym of M. meretrix.
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