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邓思平, 陈松林, 刘本伟. 2008: 半滑舌鳎FTZ-F1 cDNA克隆及表达分析(英文). 动物学研究, 29(6): 592-598. DOI: 10.3724/SP.J.1141.2008.06592
引用本文: 邓思平, 陈松林, 刘本伟. 2008: 半滑舌鳎FTZ-F1 cDNA克隆及表达分析(英文). 动物学研究, 29(6): 592-598. DOI: 10.3724/SP.J.1141.2008.06592
DENG Si-ping, CHEN Song-lin, LIU Ben-wei. 2008: Molecular Cloning and Expression Analysis of FTZ-F1 in the Half-smooth Tongue-sole, Cynoglossus semilaevis. Zoological Research, 29(6): 592-598. DOI: 10.3724/SP.J.1141.2008.06592
Citation: DENG Si-ping, CHEN Song-lin, LIU Ben-wei. 2008: Molecular Cloning and Expression Analysis of FTZ-F1 in the Half-smooth Tongue-sole, Cynoglossus semilaevis. Zoological Research, 29(6): 592-598. DOI: 10.3724/SP.J.1141.2008.06592

半滑舌鳎FTZ-F1 cDNA克隆及表达分析(英文)

Molecular Cloning and Expression Analysis of FTZ-F1 in the Half-smooth Tongue-sole, Cynoglossus semilaevis

  • 摘要: 为研究性别相关基因FTZ-F1在半滑舌鳎鱼中的表达特征,采用同源克隆策略,从其精巢分离了3 143 bp长的半滑舌鳎FTZ-F1(hsFTZ-F1)的全长cDNA,该序列包含1 458 bp开放阅读框,66 bp长的5’末端非编码区(UTR),1 619 bp长的3’末端UTR。mRNA的组织分布、氨基酸序列和系统发生分析表明:hsFTZ-F1属于SF-1/Ad4BP类群。RT-PCR分析表明:hsFTZ-F1 mRNA的分布广泛,几乎在所有组织都有表达,但在性腺、肾脏、脑和头肾组织中表达最强,其他组织表达较弱,雌鱼脑和头肾中的表达量明显高于雄性。胚胎发育过程中表达量都高于孵化后仔鱼的表达量,表明hsFTZ-F1可能参与了半滑舌鳎的器官形成过程。

     

    Abstract: To investigate the expression characteristics of sex related gene of FTZ-F1 in the half-smooth tongue-sole (Cynoglossus semilaevis), the homologue FTZ-F1 (hsFTZ-F1) full-length cDNA was isolated from the testis by homologous cloning, and the cDNA included the open reading frame and a 66 bp 5'-UTR, along with a 1 619 bp 3'-UTR, encoding a predicted 485 amino acid protein. Sequence, tissue distribution and phylogenic analyses of the FTZ-F1 showed that the hsFTZ-F1 belonged to SF-1/Ad4BP group. The hsFTZ-F1 transcripts were highly abundant in the gonads, kidneys, brain and head-kidneys, but weakly in other tissues. However, the expression level in the brain and head-kidney of female was highly abundant than in the male. The hsFTZ-F1 expression was highly abundant in the embryo than in the larvae, which suggested that the hsFTZ-F1 may be involved in the organogenesis in the tongue sole.

     

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