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刘树柏, 何英英, 钱金桥, 张云. 2008: 电镜分析非晶状体晶状体蛋白与三叶因子蛋白复合物在人红细胞膜上的孔道形成效应. 动物学研究, 29(5): 503-510. DOI: 10.3724/SP.J.1141.2008.05503
引用本文: 刘树柏, 何英英, 钱金桥, 张云. 2008: 电镜分析非晶状体晶状体蛋白与三叶因子蛋白复合物在人红细胞膜上的孔道形成效应. 动物学研究, 29(5): 503-510. DOI: 10.3724/SP.J.1141.2008.05503
LIU Shu-bai, HE Ying-ying, QIAN Jin-qiao, ZHANG Yun. 2008: Electron Microscopic Analysis of CAT Pore-orming Effect on Human Erythrocyte Membrane. Zoological Research, 29(5): 503-510. DOI: 10.3724/SP.J.1141.2008.05503
Citation: LIU Shu-bai, HE Ying-ying, QIAN Jin-qiao, ZHANG Yun. 2008: Electron Microscopic Analysis of CAT Pore-orming Effect on Human Erythrocyte Membrane. Zoological Research, 29(5): 503-510. DOI: 10.3724/SP.J.1141.2008.05503

电镜分析非晶状体晶状体蛋白与三叶因子蛋白复合物在人红细胞膜上的孔道形成效应

Electron Microscopic Analysis of CAT Pore-orming Effect on Human Erythrocyte Membrane

  • 摘要: 非晶状体βγ晶状体蛋白与三叶因子蛋白复合物(non-lens βγ-crystallin and trefoil factor complex,βγ-CAT)是一个从大蹼铃蟾 (Bombina maxima) 皮肤分泌物中分离的天然分子量为72 kDa的全新的蛋白复合物。本研究测定了βγ-CAT处理红细胞后引起细胞内钾离子外流与溶血效应的时效曲线,结合扫描电子显微镜和透射电子显微镜分析βγ-CAT处理红细胞引起的早期形态学变化。结果表明:βγ-CAT(3 nmol/L)37℃处理红细胞5 min,(93.31±5.89)%的细胞内钾离子迅速外流(P<0.01),相应溶解率为(13.12 

    Abstract: βγ-CAT is a naturally existing multifunctional protein complex of non-lens βγ-crystallin and trefoil factor from Bombina maxima skin secretions. In this paper, we first analysed the time-response curve of hemolysis and intracellular potassium efflux induced by βγ-CAT on human erythrocytes. After βγ-CAT (3 nmol/L) in human erythrocytes was treated at 37℃ for 5 min, we found that about 93.31±5.89% (P<0.01) of the intracellular potassium ions leaked, whereas the percentage of hemolysis was only13.12±1.92 % (P<0.05). We employed electron microscopy to observe the erythrocytes’ morphological changes and found erythrocytes were swollen and some had globular structures occurring on the cell surfaces, and fast releasing hemoglobins. These results indicated that the hemolytic effect of βγ-CAT on human erythrocyte membranes was due to intracellular potassium ion’s rapid efflux. These results demonstrate morphological proof in understanding the mechanism of βγ-CAT pore-forming effect on human erythrocytes.