Abstract: In the present study, the effects of fluorocitrate on the proliferation of cultured glioblastomas G422 cells were investigated. Proliferation of glioblastomas G422 cells was measured by MTT assay at 36 h, 48 h and 60 h after fluorocitrate (0.0025 mmol/L, 0.005 mmol/L, 0.01 mmol/L, 0.025 mmol/L and 0.1 mmol/L) treatment. Our results showed that: (1) Fluorocitrate inhibited the proliferation of glioblastomas G422 cells in a dose dependent manner; (2) The inhibition rate increased with treatment time at high concentrations of fluorocitrate (0.01 mmol/L, 0.025 mmol/L and 0.1 mmol/L); (3) The inhibition rate did not increase with treatment time at low concentrations of fluorocitrate (0.0025 mmol/L and 0.005 mmol/L). This study indicated that fluorocitrate inhibited the proliferation of glioma cells as a function of fluorocitrate concentration and treatment time.