Effects of Vitamin E on Reproduction Endocrine and Ovary Structures in Mice Acutely Treated with TCDD
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摘要: 为研究维生素E对2，3，7，8－四氯二苯并二噁英（TCDD）染毒小鼠生殖内分泌功能和卵巢的影响，设计了急性染毒实验。将24只4－5周龄的健康雌性小鼠（Mus musculus）随机分为对照组、TCDD(30 µg/kg)染毒组和染毒同时给予100 mg/kg维生素E组3组。通过放射免疫法测定小鼠血浆孕酮、雌二醇含量的变化，显微镜下计数卵泡数量并观察其形态变化。结果表明，TCDD能导致小鼠血浆孕酮含量的降低及卵巢结构的紊乱、卵泡数量的减少。100 mg/kg的维生素E能明显对抗急性TCDD染毒引起的雌鼠孕酮含量和卵泡数量降低，缓解TCDD引起的小鼠卵巢形态结构的病变；TCDD染毒和维生素E对小鼠血浆雌二醇含量均没有明显影响。结果表明，维生素E对TCDD引起的内分泌功能紊乱以及卵泡数量降低有拮抗作用。Abstract: The study was conducted to investigate the effects of vitamin E (VE) on reproduction endocrine and ovary structures in mice acutely treated with TCDD. Twenty-four mice were divided into control groups, TCDD-treated with 30 µg/kg group, and a TCDD-treated with 30 μg/kg followed with 100 mg/kg vitamin E group. The level of progesterone and estradiol of plasma were analyzed by radio-immunity methods. The number of ova and the ovary forms were counted and observed under the microscope. The results showed that TCDD obviously decreased the level of plasma progesterone and the number of ova, changed the forms of ovaries, while 100 mg/kg VE significantly alleviated these effects caused by TCDD. TCDD and VE had no significant effects on the levels of plasma estradiol. The results indicated that VE had anti-actions on the endocritic function and a decrease in the progesterone level and the ova number in mice, caused by TCDD.
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Key words:
- TCDD /
- Vitamin E /
- Reproduction /
- Endocrine
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