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杨志明, 闫阳阳, 吴勇, 于娜, 刘泽文. 2023: EcR/USP-1介导蜕皮激素信号调控拟环纹豹蛛的生长发育和生殖. 动物学研究, 44(1): 43-52. DOI: 10.24272/j.issn.2095-8137.2022.282
引用本文: 杨志明, 闫阳阳, 吴勇, 于娜, 刘泽文. 2023: EcR/USP-1介导蜕皮激素信号调控拟环纹豹蛛的生长发育和生殖. 动物学研究, 44(1): 43-52. DOI: 10.24272/j.issn.2095-8137.2022.282
Zhi-Ming Yang, Yang-Yang Yan, Yong Wu, Na Yu, Ze-Wen Liu. 2023: EcR/USP-1-mediated ecdysteroid signaling regulates wolf spider (Pardosa pseudoannulata) development and reproduction. Zoological Research, 44(1): 43-52. DOI: 10.24272/j.issn.2095-8137.2022.282
Citation: Zhi-Ming Yang, Yang-Yang Yan, Yong Wu, Na Yu, Ze-Wen Liu. 2023: EcR/USP-1-mediated ecdysteroid signaling regulates wolf spider (Pardosa pseudoannulata) development and reproduction. Zoological Research, 44(1): 43-52. DOI: 10.24272/j.issn.2095-8137.2022.282

EcR/USP-1介导蜕皮激素信号调控拟环纹豹蛛的生长发育和生殖

EcR/USP-1-mediated ecdysteroid signaling regulates wolf spider (Pardosa pseudoannulata) development and reproduction

  • 摘要: 生殖期的狼蛛科雌蛛通过携带卵袋和幼蛛表现出细致的母性照料。在节肢动物的生长发育和生殖调控中,蜕皮激素受体EcR和超气门蛋白USP等核受体因介导蜕皮激素信号而备受关注。该研究在捕食性狼蛛——拟环纹豹蛛基因组中共鉴定到23个核受体基因,包括1个EcR和2个USP。采用RNA干扰技术降低EcRUSP-1的表达会显著抑制幼蛛的生长发育和导致雌蛛产下的卵无法存活。只有EcRUSP-1可以响应蜕皮激素变化,并且当蜘蛛蜕皮激素合成受阻时,会引起与EcRUSP-1下调表达时相似的表型。该结果表明EcR/USP-1介导蜕皮激素信号调控拟环纹豹蛛的生长发育和生殖。进一步研究发现,雌蛛会取食因蜕皮激素信号受阻而产生的无法存活的卵,此举将首个生殖周期缩短了7.19天。终止无效的生殖使蜘蛛更快地产生新的卵袋。该生殖策略可以在一定程度上挽救因卵无法存活而导致的种群数量亏损和补偿母性照料的生理损耗,这将有益于拟环纹豹蛛种群的保护及其对害虫的自然控制。

     

    Abstract: Lycosidae females demonstrate meticulous maternal care of offspring by carrying egg sacs and juvenile spiderlings during the reproductive stage. Nuclear receptors (NRs), especially the ecdysone receptor (EcR) and ultraspiracle (USP), have attracted considerable attention in the regulation of arthropod development and reproduction due to their pivotal roles in ecdysteroid signaling cascades. In the present study, 23 NRs, including one EcR and two USPs, were identified in the genome of the predatory wolf spider Pardosa pseudoannulata. RNA interference (RNAi) targeting EcR and USP-1 inhibited spiderling development and resulted in non-viable eggs in the egg sacs. EcR and USP-1 responded to changes in ecdysteroid levels, and interference in ecdysteroid biosynthesis led to similar phenotypes as dsEcR and dsUSP-1 treatments. These findings suggest that EcR/USP-1-mediated ecdysteroid signaling regulates P. pseudoannulata development and reproduction. The P. pseudoannulata females with suppressed ecdysteroid signaling proactively consumed their non-viable egg sacs, resulting in a 7.19 d shorter first reproductive cycle than the controls. Termination of the failed reproductive cycle enabled the spiders to produce a new egg sac more rapidly. This reproductive strategy may partially rescue the reduction in population growth due to non-viable eggs and compensate for the physiological expenditure of wasted maternal care, which would be beneficial for the conservation of P. pseudoannulata populations and their natural control of insect pests.

     

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